DETECTION OF POLYGALACTURONASE, PECTIN-LYASE AND PECTIN-ESTERASE ACTIVITIES IN A SACCHAROMYCES-CEREVISIAE STRAIN

被引：84
作者：
GAINVORS, A
FREZIER, V
LEMARESQUIER, H
LEQUART, C
AIGLE, M
BELARBI, A
机构：
[1] UNIV REIMS, FAC SCI, MICROBIOL GEN LAB, F-51062 REIMS, FRANCE
[2] UNIV BORDEAUX 2, GENET LAB, F-33405 TALENCE, FRANCE
来源：
YEAST | 1994年 / 10卷 / 10期
关键词：
PECTINASE; POLYGALACTURONASE; PECTINLYASE; PECTINESTERASE; YEAST; SACCHAROMYCES CEREVISIAE;
D O I：
10.1002/yea.320101008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The catalytic capacity of several excreted pectinolytic enzymes obtained from various yeast strains was examined using in vivo and biochemical techniques. Of the 33 yeast strains studied, 30 were isolated from champagne wine during alcoholic fermentation. Only one yeast strain was found to excrete pectinolytic enzymes and was identified as Saccharomyces cerevisisae and designated SCPP. Pulsed-field gel electrophoresis and the polymerase chain reaction technique were used to characterize further this specific strain. Three types of pectinolytic enzymes were found to be excreted by SCPP: polygalacturonase, pectin-lyase and pectin-esterase. These enzymes allow pectin hydrolysis during cell growth.
引用
收藏
页码：1311 / 1319
页数：9
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