SPECIFIC TRANSPORT OF INORGANIC-PHOSPHATE AND C-3-SUGAR PHOSPHATE AND C-6-SUGAR-PHOSPHATE ACROSS THE ENVELOPE MEMBRANES OF TOMATO (LYCOPERSICON-ESCULENTUM) LEAF-CHLOROPLASTS, TOMATO FRUIT-CHLOROPLASTS AND FRUIT-CHROMOPLASTS
LYCOPERSICON ESCULENTUM;
LEAF CHLOROPLAST;
FRUIT CHLOROPLAST;
FRUIT CHROMOPLAST;
ENVELOPE MEMBRANE;
PHOSPHATE TRANSLOCATOR;
RECONSTITUTION;
D O I:
10.1111/j.1438-8677.1994.tb00821.x
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Plastidial envelope membranes were isolated from tomato (Lycopersicon esculentum) leaves and green and red tomato fruits by isopycnic discontinuous sucrose density gradient centrifugation. Solubilized envelope membrane proteins were reconstituted into liposomes. Transport measurements revealed that the phosphate translocator from tomato leaves transports inorganic phosphate, 3-phosphoglycerate and triosephosphates. The phosphate translocators of green and red fruit plastids catalyze, in addition to the transport of these substrates, also the transport of glucose-6-phosphate, glucose-1-phosphate and phosphoenolpyruvate.