PURIFICATION, CHARACTERIZATION, GENE CLONING, AND SEQUENCING OF A NEW BETA-GLUCOSIDASE FROM BACILLUS-CIRCULANS SUBSP ALKALOPHILUS

被引：48
|
作者：
PAAVILAINEN, S [1 ]
HELLMAN, J [1 ]
KORPELA, T [1 ]
机构：
[1] CTR BIOTECHNOL,SF-20521 TURKU,FINLAND
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 03期
关键词：
D O I：
10.1128/AEM.59.3.927-932.1993
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated M(r) of 51,303. The enzyme exhibited from 45 to 66% identity with five bacterial beta-glucosidases.
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收藏
页码：927 / 932
页数：6
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