DETERMINATION OF BOVINE BUTTERFAT TRIACYLGLYCEROLS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY AND GAS-CHROMATOGRAPHY

被引：30
|
作者：
MANIONGUI, C [1 ]
GRESTI, J [1 ]
BUGAUT, M [1 ]
GAUTHIER, S [1 ]
BEZARD, J [1 ]
机构：
[1] FAC SCI MIRANDE,PHYSIOL ANIM NUTR LAB,BP 138,F-21004 DIJON,FRANCE
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 543卷 / 01期
关键词：
D O I：
10.1016/S0021-9673(01)95757-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Triacylglycerols (TGs) from a sample of summer butterfat (bovine milk) were analysed and fractionated by reversed-phase liquid chromatography (RPLC). Fatty acid and TG compositions of each of the 47 RPLC fractions ranging from 0.1 tot 6.9% were determined by capillary gas chromatography. The data were used together to determine the quantitative composition of the molecular species of TGs. A large number of TG species, accounting for 80% of the total, could be unequivocally identified and individually determined. The combination of the chromatographic methods used proved to be a powerful and accurate approach for the determination of molecular species of TGs in a complex fat, but also a difficult and time-consuming task.
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页码：81 / 103
页数：23
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