A NUCLEAR CAP-BINDING PROTEIN COMPLEX INVOLVED IN PRE-MESSENGER-RNA SPLICING

被引：447
作者：
IZAURRALDE, E
LEWIS, J
MCGUIGAN, C
JANKOWSKA, M
DARZYNKIEWICZ, E
MATTAJ, IW
机构：
[1] UNIV WARSAW,DEPT CHEM,PL-02093 WARSAW,POLAND
[2] UNIV WARSAW,DEPT BIOPHYS,PL-02089 WARSAW,POLAND
来源：
CELL | 1994年 / 78卷 / 04期
关键词：
D O I：
10.1016/0092-8674(94)90530-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cap-binding protein complex (CBC) present in the nuclei of HeLa cells has been characterized. Purified CBC consists of two previously identified proteins, CBP80 and CBP20. These proteins are shown to cofractionate to apparent homogeneity and to be coimmunoprecipitable with anti-CBP80 antibodies, Analysis of the inhibition of pre-mRNA splicing in vitro and in vivo by chemically modified analogs of the cap structure, and of the binding of these analogs to CBC in vitro, suggests a role for the complex in splicing. Extracts immunodepleted of CBC do not efficiently splice an adenoviral pre-mRNA owing to blockage of an early step in splicing complex formation. CBC may therefore play a role in pre-mRNA recognition.
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页码：657 / 668
页数：12
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