G-PROTEINS AND ADENYLYL-CYCLASE IN OVARIAN GRANULOSA-CELLS OF AMAGO SALMON (ONCORHYNCHUS-RHODURUS)

被引:0
作者
MITA, M
YOSHIKUNI, M
NAGAHAMA, Y
机构
[1] NATL INST BASIC BIOL,REPROD BIOL LAB,OKAZAKI,AICHI 444,JAPAN
[2] TEIKYO JR COLL,SHIBUYA KU,TOKYO 151,JAPAN
关键词
G-PROTEIN; ADENYLYL CYCLASE; GRANULOSA CELL; AMAGO SALMON; GONADOTROPIN; STEROIDOGENESIS;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The involvement of guanine nucleotide-binding regulatory proteins (G-proteins) and adenylyl cyclase in the gonadotropin stimulation of cAMP was investigated using crude membrane fractions from granulosa cells of amago salmon (Oncorhynchus rhodurus) postvitellogenic ovarian follicles. Although cholera toxin-catalyzed ADP ribosylation occurred in 45- and 50-kDa proteins, only the former was recognized by an antibody against the alpha-subunit of Gs. With pertussis toxin, only the 41-kDa protein was ADP-ribosylated. This 41-kDa protein was recognized by an antibody against the alpha-subunit of Gi. Partially purified chum salmon gonadotropin (SGA) stimulated adenylyl cyclase activity in crude membrane preparations of granulosa cells only in the presence of pertussis toxin in the incubation medium. Adenosine inhibited adenylyl cyclase in the presence of GTP and pertussis toxin reversed it. Unlike SGA, forskolin, which acts upon adenylyl cyclase without G-protein interaction, markedly stimulated adenylyl cyclase activity in the absence of pertussis toxin. These results provide evidence that both stimulatory (Gs) and inhibitory (Gi) regulation by adenylyl cyclase operates in the granulosa cells of amago salmon postvitellogenic ovarian follicles. It is possible that, although a stimulatory receptor interacts with Gs, its activity is influenced by the functional state of Gi.
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页码:83 / 88
页数:6
相关论文
共 25 条
[1]   STIMULATION INVITRO OF ESTRADIOL SECRETION BY THE OVARY OF A CICHLID FISH, SAROTHERODON-AUREUS [J].
BOGOMOLNAYA, A ;
YARON, Z .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1984, 53 (02) :187-196
[2]   THE MODE OF ACTION OF CARP GONADOTROPIN ON THE STIMULATION OF ANDROGEN PRODUCTION BY CARP TESTIS INVITRO [J].
CHANG, YS ;
HUANG, FL .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1982, 48 (02) :147-153
[3]  
FAIN JN, 1972, J BIOL CHEM, V247, P6866
[4]  
GILL DM, 1982, ADP RIBOSYLATION REA, P593
[5]   G-PROTEINS AND DUAL CONTROL OF ADENYLATE-CYCLASE [J].
GILMAN, AG .
CELL, 1984, 36 (03) :577-579
[6]   ULTRASENSITIVE METHOD FOR SIMULTANEOUS DETERMINATION OF CYCLIC-AMP AND CYCLIC-GMP IN SMALL-VOLUME SAMPLES FROM BLOOD AND TISSUE [J].
HONMA, M ;
SATOH, T ;
TAKEZAWA, J ;
UI, M .
BIOCHEMICAL MEDICINE, 1977, 18 (03) :257-273
[7]   ESTRADIOL-17-BETA PRODUCTION IN AMAGO SALMON (ONCORHYNCHUS-RHODURUS) OVARIAN FOLLICLES - ROLE OF THE THECAL AND GRANULOSA-CELLS [J].
KAGAWA, H ;
YOUNG, G ;
ADACHI, S ;
NAGAHAMA, Y .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1982, 47 (04) :440-448
[8]   INVOLVEMENT OF 3',5'-CYCLIC ADENOSINE-MONOPHOSPHATE IN THE CONTROL OF FOLLICULAR STEROIDOGENESIS OF AMAGO SALMON (ONCORHYNCHUS-RHODURUS) [J].
KANAMORI, A ;
NAGAHAMA, Y .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1988, 72 (01) :39-53
[9]   DEVELOPMENTAL-CHANGES IN THE PROPERTIES OF GONADOTROPIN RECEPTORS IN THE OVARIAN FOLLICLES OF AMAGO SALMON (ONCORHYNCHUS-RHODURUS) DURING OOGENESIS [J].
KANAMORI, A ;
NAGAHAMA, Y .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1988, 72 (01) :25-38
[10]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+