DEUTERIUM-OXIDE AS A TOOL FOR STUDY OF AMINO-ACID METABOLISM

被引：23

作者：

MITRA, R ^{[1
]}

BURTON, J ^{[1
]}

VARNER, JE ^{[1
]}

机构：

[1] WASHINGTON UNIV, DEPT BIOL, ST LOUIS, MO 63130 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1976年 / 70卷 / 01期

关键词：

D O I：

10.1016/S0003-2697(76)80042-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Deuterium oxide in nontoxic concentrations was used to study, in intact [Hordeum vulgare] seedlings, the biosynthesis of amino acids. The extent and pattern of deuteration, as determined by a GLC-mass spectrometer system, permits conclusions about the biosynthesis of individual amino acids and also about their exposure to transaminases and other enzymes that might introduce deuterium into specific positions of the amino acid by exchange. This method could be used to study amino acid biogenesis in any organism that can tolerate 20-40% deuterium oxide for a period of a few hours to a few days.

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页码：1 / 17

页数：17

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