VASOACTIVE INTESTINAL POLYPEPTIDE AND NITRIC-OXIDE SYNTHASE DISTRIBUTION IN THE ENTERIC PLEXUSES OF THE HUMAN COLON - AN HISTOCHEMICAL-STUDY AND QUANTITATIVE-ANALYSIS

被引：20

作者：

MATINI, P

FAUSSONEPELLEGRINI, MS

CORTESINI, C

MAYER, B

机构：

[1] UNIV FLORENCE, DEPT HUMAN ANAT & HISTOL, HISTOL SECT, I-50134 FLORENCE, ITALY

[2] UNIV FLORENCE, POLICLIN CAREGGI, SURG CLIN, I-50134 FLORENCE, ITALY

[3] GRAZ UNIV, INST PHARMACOL & TOXICOL, GRAZ, AUSTRIA

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 1995年 / 103卷 / 06期

关键词：

D O I：

10.1007/BF01457541

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Vasoactive intestinal polypeptide (VIP) and nitric oxide synthase (NOS) positive innervation patterns were immunohistochemically and statistically evaluated in the human colon. Specimens from the right colon (cecum, ascending and right transverse colon) and left colon (left transverse and descending colon) were obtained surgically, fixed either in paraformaldehyde or in Carnoy's or in Bouin's, and paraffin embedded. Sections were stained with hematoxylin-eosin, toluidine blue, cresyl violet, neuron-specific enolase, anti-VIP, and anti-NOS. The same results were obtained regardless of the fixative used. Enolase-positive, VIP-positive, and NOS-positive cells were occasionally found within the circular muscle and interpreted as neurons. VIP-positive nerve fibers were evenly distributed within the circular muscle while NOS-positive ones were lacking in its inner portion. The left colon was richer in neurons than the right colon, at both plexuses. VIP- and NOS-positive neuron densities were higher at the left than at the right colon, whereas at all colonic levels VIP-positive neuron percentages at both plexuses and NOS-positive ones at the myenteric plexus were similar. At the submucous plexus the NOS-positive neuron percentage was lower than that of the VIP-positive one. In conclusion: (a) the right colon contains a lower number of neurons and of VIP- and NOS-positive ones than the left colon, and (b) VIP- and NOS-positive fibers are differently distributed in the in ner and outer portions of the circular muscle.

引用

页码：415 / 423

页数：9

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