DELETION ANALYSIS OF A PHYTOCHROME-REGULATED MONOCOT RBCS PROMOTER IN A TRANSIENT ASSAY SYSTEM

被引：28

作者：

ROLFE, SA ^{[1
]}

TOBIN, EM ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL,405 HILGARD AVE,LOS ANGELES,CA 90024

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 07期

关键词：

LEMNA-GIBBA; PARTICLE BOMBARDMENT; RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE; OXYGENASE;

D O I：

10.1073/pnas.88.7.2683

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have developed a transient gene expression assay system in the aquatic monocot Lemna gibba in which DNA was introduced into intact tissue by particle bombardment. Constructs based on the Lemna rbcS gene SSU5B, which is positively regulated by phytochrome in vivo, also showed phytochrome regulation in the transient assay system. Reporter gene expression increased 12-fold over dark levels in response to a single treatment with red light. This increase was not observed if far-red light was immediately followed by the red light. A 5' deletion analysis of the promoter defined a region from position -205 to position -83 relative to the start of transcription as necessary to observe the phytochrome response. This region contains the binding site for the light-induced binding activity (LRF-1) found in Lemna nuclear extracts. Upstream of position -205, we found evidence for the presence of at least two upstream activating sequences and a silencer.

引用

页码：2683 / 2686

页数：4

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