FINE-STRUCTURE OF A NEW HUMAN MICROSPORIDIAN, ENCEPHALITOZOON-HELLEM, IN CULTURE

被引:61
作者
DIDIER, PJ
DIDIER, ES
ORENSTEIN, JM
SHADDUCK, JA
机构
[1] TULANE UNIV,TULANE REG PRIMATE RES CTR,DEPT MICROBIOL,COVINGTON,LA 70433
[2] GEORGE WASHINGTON UNIV,MED CTR,DEPT PATHOL,WASHINGTON,DC 20037
[3] TEXAS A&M UNIV SYST,COLL VET MED,COLLEGE STN,TX 77843
来源
JOURNAL OF PROTOZOOLOGY | 1991年 / 38卷 / 05期
关键词
AIDS; ENCEPHALITOZOONOSIS; MICROSPORIDIOSIS; OPPORTUNISTIC PARASITE; ULTRASTRUCTURE;
D O I
10.1111/j.1550-7408.1991.tb04824.x
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Encephalitozoon hellem is a new human microsporidian isolated from corneal biopsies and conjunctival scrapings of three AIDS patients and cultured in Madin Darby canine kidney (MDCK) cells. Encephalitozoon hellem and Encephalitozoon cuniculi display different protein profiles with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and unique antibody binding patterns with murine antisera against Western blots of each organism. Developmental stages of E. hellem in culture are similar to E. cuniculi. Meronts are 1.3-2.7-mu-m in diameter, develop within a parasitophorous vacuole adjacent to the vacuolar membrane, divide by binary fission, and contain one or two discrete nuclei. Sporonts measure 2 x 3-mu-m, separate from the vacuolar membrane, and have a thickened outer membrane. Sporoblasts display a tri-layered wall and possess the earliest recognized polar filaments. Mature spores measure 1 x 1.5-mu-m and are more electron-dense than other stages. Each spore contains a single nucleus, a polar tubule with four to nine coils, thin electron-dense exospore and thick, electron-lucent endospore. Although E. hellem and E. cuniculi differ biochemically and immunologically, their fine structure and development are indistinguishable.
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页码:502 / 507
页数:6
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