THE REGULATION OF FATTY-ACID CHAIN ELONGATION IN RAT-LIVER MICROSOMES - ROLE OF FASTING AND COASH

被引：3
作者：
REICHELT, WH [1 ]
GRAV, HJ [1 ]
CHRISTIANSEN, EN [1 ]
机构：
[1] UNIV OSLO,INST NUTR RES,N-0316 OSLO 3,NORWAY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1214卷 / 02期
关键词：
FATTY ACID ELONGATION; COASH; FASTING; MICROSOME; ENZYME KINETICS; (RAT); (LIVER);
D O I：
10.1016/0005-2760(94)90033-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The elongation system for palmitic acid in rat liver microsomes was decreased to 1/3 by fasting, while the elongation of eicosapentaenoic acid was not sensitive to fasting. The rate of eicosapentaenoic acid elongation in the fed state was 50% higher than using palmitic acid as a substrate. The saturated and polyunsaturated fatty acyl-CoA substrates exhibited positive cooperativity on the rate-limiting condensing step in the elongation system, with a Hill constant of approx. 2. An inhibition by CoASH on the total elongation reaction as well as on the condensation step was demonstrated using acyl-CoA substrates, and followed a hyperbolic pattern. The concentrations giving a 50% inhibition (30-70 mu M) were in the range found in rat hepatocyte cytosol, indicating that free CoASH has the potential to act as a physiological regulator.
引用
收藏
页码：109 / 114
页数：6
相关论文
共 41 条
[21]   EFFECTS OF FREE FATTY-ACID ON HEPATIC AMINO-ACID-METABOLISM IN ISOLATED PERFUSED-RAT-LIVER [J].
DEBANDT, JP ;
CYNOBER, L ;
REY, C ;
COUDRAYLUCAS, C ;
LIM, SK ;
POUPON, R ;
GIBOUDEAU, J .
GASTROENTEROLOGIE CLINIQUE ET BIOLOGIQUE, 1992, 16 (01) :46-49
[22]   FATTY-ACID ELONGATION IN THE BIOSYNTHESIS OF (Z)-10-HEPTADECEN-2-ONE AND 2-TRIDECANONE IN EJACULATORY BULB MICROSOMES OF DROSOPHILA-BUZZATII [J].
SKIBA, PJ ;
JACKSON, LL .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1994, 24 (08) :847-853
[23]   EFFECT OF VARIOUS N-3/N-6 FATTY-ACID RATIO CONTENTS OF HIGH-FAT DIETS ON RAT-LIVER AND HEART PEROXISOMAL AND MITOCHONDRIAL BETA-OXIDATION [J].
VAMECQ, J ;
VALLEE, L ;
DELAPORTE, PL ;
FONTAINE, M ;
DECRAEMER, D ;
VANDENBRANDEN, C ;
LAFONT, H ;
GRATAROLI, R ;
NALBONE, G .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1170 (02) :151-156
[24]   Fasting enriches liver triacylglycerol with n-3 polyunsaturated fatty acids: implications for understanding the adipose-liver axis in serum docosahexaenoic acid regulation [J].
Marks, Kristin A. ;
Marvyn, Phillip M. ;
Henao, Juan J. Aristizabal ;
Bradley, Ryan M. ;
Stark, Ken D. ;
Duncan, Robin E. .
GENES AND NUTRITION, 2015, 10 (06)
[25]   A COMPARATIVE-STUDY OF THE CONFORMATIONAL PROPERTIES OF ESCHERICHIA-COLI-DERIVED RAT INTESTINAL AND LIVER FATTY-ACID BINDING-PROTEINS [J].
MUGA, A ;
CISTOLA, DP ;
MANTSCH, HH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1162 (03) :291-296
[26]   LOW FATTY-ACID ELONGATION RATE IN THE PRESENCE OF NADH IN THE LIVER ENDOPLASMIC-RETICULUM - OVERINHIBITION BY BSA AT THE BETA-KETOREDUCTASE LEVEL [J].
DEMIRKAPI, N ;
GHESQUIER, D .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1046 (02) :229-232
[27]   SEX DIFFERENCE IN TRIGLYCERIDE FATTY-ACID SUBSTRATE CYCLING OF RAT ADIPOSE-TISSUE - INDIRECT REGULATION BY ANDROGENS [J].
HANSSON, P ;
SAGGERSON, D ;
NILSSONEHLE, P .
HORMONE AND METABOLIC RESEARCH, 1991, 23 (10) :465-468
[28]   Indirect dexamethasone down-regulation of the liver fatty acid-binding protein expression in rat liver [J].
Foucaud, L ;
Niot, I ;
Kanda, T ;
Besnard, P .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1998, 1391 (02) :204-212
[29]   Stimulation of rat-liver branched-chain α-keto acid dehydrogenase activity by chronic metabolic acidosis [J].
Rodríguez-Bayona, B ;
Peragón, J .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1998, 30 (04) :529-534
[30]   INSULIN RESTORES FATTY-ACID COMPOSITION EARLIER IN LIVER-MICROSOMES THAN ERYTHROCYTE-MEMBRANES IN STREPTOZOTOCIN-INDUCED DIABETIC RATS [J].
SHIN, CS ;
LEE, MK ;
PARK, KS ;
KIM, SY ;
CHO, BY ;
LEE, HK ;
KOH, CS ;
MIN, HK .
DIABETES RESEARCH AND CLINICAL PRACTICE, 1995, 29 (02) :93-98
12345