PHYSICAL CHARACTERIZATION OF THE ESCHERICHIA-COLI-B GENE ENCODING NITROREDUCTASE AND ITS OVER-EXPRESSION IN ESCHERICHIA-COLI K12

被引：52

作者：

MICHAEL, NP ^{[1
]}

BREHM, JK ^{[1
]}

ANLEZARK, GM ^{[1
]}

MINTON, NP ^{[1
]}

机构：

[1] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,DEPT MOLEC MICROBIOL,DIV RES,SALISBURY SP4 0JG,WILTS,ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 124卷 / 02期

关键词：

ESCHERICHIA COLI; NITROREDUCTASE; NUCLEOTIDE SEQUENCE;

D O I：

10.1111/j.1574-6968.1994.tb07284.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Escherichia coli B gene (nfnB) encoding nitroreductase has been cloned in Escherichia coli K-12 and its nucleotide sequence determined. The translated amino acid sequence was found to share substantial identity (88.5%) with the equivalent proteins of Enterobacter cloacae and Salmonella typhimurium. When the structural gene was placed under the transcriptional control of either the trp or lac promoter, recombinant nitroreductase was accumulated to 33% and 25% of the cell's soluble protein, respectively. Substitution of the nfrB ribosome binding site with that of the E. coli lacZ gene reduced production levels of nitroreductase. The sequenced region also contained two incomplete open reading frames of unknown function.

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页码：195 / 202

页数：8

相关论文

共 20 条

[1] THE BIOACTIVATION OF 5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE (CB1954) .1. PURIFICATION AND PROPERTIES OF A NITROREDUCTASE ENZYME FROM ESCHERICHIA-COLI - A POTENTIAL ENZYME FOR ANTIBODY-DIRECTED ENZYME PRODRUG THERAPY (ADEPT) [J].

ANLEZARK, GM ;

MELTON, RG ;

SHERWOOD, RF ;

COLES, B ;

FRIEDLOS, F ;

KNOX, RJ .

BIOCHEMICAL PHARMACOLOGY, 1992, 44 (12) :2289-2295

[2]

BREHM JK, 1991, APPL MICROBIOL BIOT, V36, P358

[3]

BRYANT C, 1991, J BIOL CHEM, V266, P4126

[4] THE PMTL NIC-CLONING VECTORS .1. IMPROVED PUC POLYLINKER REGIONS TO FACILITATE THE USE OF SONICATED DNA FOR NUCLEOTIDE SEQUENCING [J].

CHAMBERS, SP ;

PRIOR, SE ;

BARSTOW, DA ;

MINTON, NP .

GENE, 1988, 68 (01) :139-149

[5] THE BIOACTIVATION OF 5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE (CB1954) .2. A COMPARISON OF AN ESCHERICHIA-COLI NITROREDUCTASE AND WALKER DT DIAPHORASE [J].

KNOX, RJ ;

FRIEDLOS, F ;

SHERWOOD, RF ;

MELTON, RG ;

ANLEZARK, GM .

BIOCHEMICAL PHARMACOLOGY, 1992, 44 (12) :2297-2301

[6] A NEW CYTO-TOXIC, DNA INTERSTRAND CROSSLINKING AGENT, 5-(AZIRIDIN-1-YL)-4-HYDROXYLAMINO-2-NITROBENZAMIDE, IS FORMED FROM 5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE (CB-1954) BY A NITROREDUCTASE ENZYME IN WALKER CARCINOMA-CELLS [J].

KNOX, RJ ;

FRIEDLOS, F ;

JARMAN, M ;

ROBERTS, JJ .

BIOCHEMICAL PHARMACOLOGY, 1988, 37 (24) :4661-4669

[7] BIOACTIVATION OF CB 1954 - REACTION OF THE ACTIVE 4-HYDROXYLAMINO DERIVATIVE WITH THIOESTERS TO FORM THE ULTIMATE DNA DNA INTERSTRAND CROSS-LINKING SPECIES [J].

KNOX, RJ ;

FRIEDLOS, F ;

MARCHBANK, T ;

ROBERTS, JJ .

BIOCHEMICAL PHARMACOLOGY, 1991, 42 (09) :1691-1697

[8] THE BIOACTIVATION OF CB-1954 AND ITS USE AS A PRODRUG IN ANTIBODY-DIRECTED ENZYME PRODRUG THERAPY (ADEPT) [J].

KNOX, RJ ;

FRIEDLOS, F ;

BOLAND, MP .

CANCER AND METASTASIS REVIEWS, 1993, 12 (02) :195-212

[9] MOLECULAR-CLONING OF THE PSEUDOMONAS CARBOXYPEPTIDASE-G2 GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND PSEUDOMONAS-PUTIDA [J].

MINTON, NP ;

ATKINSON, T ;

SHERWOOD, RF .

JOURNAL OF BACTERIOLOGY, 1983, 156 (03) :1222-1227

[10] HYPOXIA-SELECTIVE ANTITUMOR AGENTS .5. SYNTHESIS OF WATER-SOLUBLE NITROANILINE MUSTARDS WITH SELECTIVE CYTOTOXICITY FOR HYPOXIC MAMMALIAN-CELLS [J].

PALMER, BD ;

WILSON, WR ;

CLIFFE, S ;

DENNY, WA .

JOURNAL OF MEDICINAL CHEMISTRY, 1992, 35 (17) :3214-3222