M(3) MUSCARINIC RECEPTORS MEDIATE AN INCREASE IN BOTH INOSITOL TRISPHOSPHATE PRODUCTION AND CYCLIC-AMP FORMATION IN DOG IRIS SPHINCTER SMOOTH-MUSCLE

被引：11

作者：

TACHADO, SD ^{[1
]}

VIRDEE, K ^{[1
]}

AKHTAR, RA ^{[1
]}

ABDELLATIF, AA ^{[1
]}

机构：

[1] MED COLL GEORGIA,DEPT BIOCHEM & MOLEC BIOL,AUGUSTA,GA 30912

来源：

JOURNAL OF OCULAR PHARMACOLOGY | 1994年 / 10卷 / 01期

关键词：

D O I：

10.1089/jop.1994.10.137

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Pharmacological studies on pirenzepine (PZ), 4-diphenylacetoxy-N-methylpiperidine (4-DAMP) and AFDX-116 antagonism of carbachol (Cch)-induced contraction, inositol trisphosphate (IP3) production and cAMP formation revealed the involvement of M(3) receptors in these responses. The PA(2) values for PZ and 4-DAMP antagonism to CCh-induced contraction were 7.1 and 9.0, respectively, and AFDX-116 had no effect on these responses. Further, 4-DAMP was a much more potent inhibitor than PZ of CCh-stimulation of IP3 production and cAMP formation. Both L-type calcium channel blockers, which inhibit Ca2+ influx, and BAPTA, an intracellular calcium chelator, inhibited these biochemical and pharmacological responses due to CCh. It is concluded that both intracellular and extracellular Ca2+ mobilization are involved in muscarinic stimulation of cAMP production, and that M(3) receptors are coupled to the activation of both phospholipase C and adenylate cyclase in this tissue. The data presented here are consistent with previous work that stimulation of muscarinic receptors in dog iris sphincter with CCh (> 5 mu M) increases intracellular cAMP levels.

引用

页码：137 / 147

页数：11

共 20 条

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