IMPROVED ENDOTHELIAL VIABILITY OF HEART-VALVES CRYOPRESERVED BY A NEW TECHNIQUE

被引：8

作者：

FENG, XJ ^{[1
]}

VANHOVE, CE ^{[1
]}

MOHAN, R ^{[1
]}

ANDRIES, L ^{[1
]}

RAMPART, M ^{[1
]}

HERMAN, AG ^{[1
]}

WALTER, PJ ^{[1
]}

机构：

[1] UNIV HOSP ANTWERP,DEPT CARDIAC SURG,WILRIJKSTR 10,B-2650 EDEGEM,BELGIUM

来源：

EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY | 1992年 / 6卷 / 05期

关键词：

AORTIC VALVE; HOMOGRAFT; PROSTACYCLIN; ENDOTHELIAL CELL; VIABILITY;

D O I：

10.1016/1010-7940(92)90107-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of this study was to compare different techniques of aortic valve cryopreservation by studying the viability of the endothelial cells. Viability was assessed by measuring their in vitro prostacyclin (PGI2) production under basal and stimulated conditions. Fresh and cryopreserved porcine valves were incubated at 37-degrees-C in tissue culture medium and PGI2 content in the medium was measured every 15 min up to 300 min. Cryopreservation by the older procedure A included 5% fetal calf serum (FCS) in the preservation medium, a plastic box inside a freezing plastic bag, a cooling schedule approximating -2-degrees-C/min, a long thawing time and few dilution steps of the cryoprotectant dimethylsulphoxide (DMSO). The newer procedure B differed from A in packaging, freezing and thawing rates and DMSO dilution. Procedure C was similar to B with the exception that FCS was omitted. Leaflets preserved by procedure A produced significantly less prostacyclin as compared to those treated according to procedures B or C. We conclude that minor differences in the cryopreservation method can become critical to endothelial functional viability.

引用

页码：251 / 255

页数：5

共 36 条

[11]

Ferrans V.J., Spray T.L., Billingham M.E., Roberts W.C., Structural changes in glutaraldehyde treated porcine heterografts used as substitute cardiac valves, Am J Cardiol, 41, pp. 1159-1184, (1978)

[12]

Gavin J.B., Monro J.L., Wall F.M., Chalcroft S.C.W., Fine structural changes in the fibroblasts of canine heart valves prepared for grafting, Thorax, 28, pp. 748-755, (1973)

[13]

Gonzalez-Lavin L., Bianchi J., Graf D., Amini S., Gordon C.I., Degenerative changes in fresh aortic root homografts in a canine model: Evidence of an immunologic influence, Transpl Proc, 20, pp. 815-819, (1988)

[14]

Gonzalez-Lavin L., Spotnitz A.J., McKenzie J.W., Gu J., Gadi I.K., Gullo J., Boyd C., Graf D., Homograft valve durability: Host or donor influence?, Heart Vessels, 5, pp. 102-106, (1990)

[15]

Heslop B.F., Wilson S.E., Hardy B.E., Antigenecity of aortic valve allografts, Ann Surg, 177, pp. 301-306, (1973)

[16]

Jaffe E.A., Cell biology of endothelial cells, Hum Pathol, 18, pp. 234-239, (1987)

[17]

Karp R.B., The future of homografts, J Cardiac Surg, 1, pp. 205-208, (1987)

[18]

Lloyd Wolfinbarger J.R., Hopkins R.A., Biology of heart valve cryopreservation, Cardiac reconstructions with allograft valves, (1989)

[19]

McGregor C.G.A., Bradley J.F., McGee J.O.D., Wheatley D.J., Tissue culture, protein and collagen synthesis in antibiotic sterilized canine heart valves, Cardiovasc Res, 10, pp. 389-393, (1976)

[20]

McNally R.T., Heacox A., Brockbank K.G.M., Method for cryopreserving heart valves, (1990)

← 1 2 3 4 →