SYNTHESIS OF N-BENZYLOXYCARBONYL-L-CYSTEINYLGLYCINE 3-DIMETHYLAMINOPROPYLAMIDE DISULFIDE - A CHEAP AND CONVENIENT NEW ASSAY FOR TRYPANOTHIONE REDUCTASE

被引：51

作者：

ELWAER, A

DOUGLAS, KT

SMITH, K

FAIRLAMB, AH

机构：

[1] UNIV MANCHESTER,DEPT PHARM,OXFORD RD,MANCHESTER M13 9PL,LANCS,ENGLAND

[2] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,LONDON WC1E 7HT,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0003-2697(91)90531-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Trypanothione disulfide [N1,N8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. Here we describe the straightforward synthesis of N,N′-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylamide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from Crithidia fasciculata and Trypanosoma cruzi. © 1991.

引用

收藏

页码：212 / 216

页数：5

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