SYNTHESIS OF N-BENZYLOXYCARBONYL-L-CYSTEINYLGLYCINE 3-DIMETHYLAMINOPROPYLAMIDE DISULFIDE - A CHEAP AND CONVENIENT NEW ASSAY FOR TRYPANOTHIONE REDUCTASE

被引：51
作者：
ELWAER, A
DOUGLAS, KT
SMITH, K
FAIRLAMB, AH
机构：
[1] UNIV MANCHESTER,DEPT PHARM,OXFORD RD,MANCHESTER M13 9PL,LANCS,ENGLAND
[2] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,LONDON WC1E 7HT,ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 01期
基金：
英国惠康基金;
关键词：
D O I：
10.1016/0003-2697(91)90531-W
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Trypanothione disulfide [N1,N8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. Here we describe the straightforward synthesis of N,N′-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylamide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from Crithidia fasciculata and Trypanosoma cruzi. © 1991.
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页码：212 / 216
页数：5
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