HETEROGENEITY WITHIN THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-2 GENE IN DIFFERENT STRAINS OF EPSTEIN-BARR-VIRUS

被引：55
作者：
AITKEN, C
SENGUPTA, SK
AEDES, C
MOSS, DJ
SCULLEY, TB
机构：
[1] UNIV PAPUA NEW GUINEA,DEPT PATHOL,PORT MORESBY,PAPUA N GUINEA
[2] ROYAL BRISBANE HOSP,DEPT PATHOL,BRISBANE,QLD,AUSTRALIA
来源：
JOURNAL OF GENERAL VIROLOGY | 1994年 / 75卷
关键词：
D O I：
10.1099/0022-1317-75-1-95
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
DNA isolated from biopsies of endemic Burkitt's lymphoma (BL) from New Guinea was analysed for the presence of Epstein-Barr virus (EBV) sequences using the polymerase chain reaction. Primers were designed to amplify sequences within the Epstein-Barr virus nuclear antigen (EBNA) 1 and 2 genes. These analyses detected the EBNA1 sequence in all the biopsies studied. Additional sets of primers directed against the EBNA2. gene were used in order to categorize the EBV strains as A-type or B-type (39 % A-type; 50 % B-type; 5 % A and B-type; 5 % untypeable). These results indicated that DNA sequence heterogeneity within the EBNA2 gene region may exist in different strains of EBV. The extent of DNA sequence heterogeneity among different strains of EBV was determined by sequencing of a region within the EBNA2 gene in a number of different A-type and B-type strains of EBV originating from Africa or New Guinea. The results demonstrated DNA sequence heterogeneity within the EBNA2 gene in different strains of EBV. This heterogeneity was more extensive among A-type strains than B-type strains of EBV.
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页码：95 / 100
页数：6
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