A RAPID POLYMERASE CHAIN-REACTION (PCR)-BASED ASSAY FOR THE IDENTIFICATION OF LISTERIA-MONOCYTOGENES IN FOOD SAMPLES

被引：63

作者：

ROSSEN, L ^{[1
]}

HOLMSTROM, K ^{[1
]}

OLSEN, JE ^{[1
]}

RASMUSSEN, OF ^{[1
]}

机构：

[1] ROYAL VET & AGR UNIV,DEPT VET MICROBIOL,FREDERIKSBERG,DENMARK

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1991年 / 14卷 / 02期

关键词：

DNA-PROBE; DETECTION; LISTERIA-MONOCYTOGENES; FOODS;

D O I：

10.1016/0168-1605(91)90101-T

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.

引用

页码：145 / 151

页数：7

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