HETEROGENEITY OF HIGH-AFFINITY UPTAKE OF L-GLUTAMATE AND L-ASPARTATE IN THE MAMMALIAN CENTRAL-NERVOUS-SYSTEM

Characteristics of high affinity uptake of L-glutamate are examined in order to evaluate the possible use of the uptake of [H-3]L-glutamate, [H-3]L-aspartate or any other suitable [H-3]-labelled substrate as a marker for glutamatergic and aspartergic synapses in autoradiographic studies in the mammalian brain. Review of data on substrate specificity indicates the presence of at least two high affinity uptake systems specific for acidic amino acids in the central nervous tissue; one which takes up L-glutamate and L-aspartate and the other which is selective for L-glutamate only. Studies on ionic requirements, too, point to the existence of at least two distinct uptake systems with high affinity for L-glutamate. The Na+-dependent uptake system(s) handle(s) both L-glutamate and L-aspartate whereas the Na+-independent uptake system(s) show(s) selectivity for L-glutamate only. Available data do not favour the Na+-dependent binding of [H-3]D-aspartate to thaw-mounted sections of frozen brain tissue as a suitable marker for glutamatergic/aspartergic synaptic nerve endings. However, there are reasons - such as the results of lesion studies and the existence of uptake sites which have a higher affinity for L-aspartate than for D-aspartate - to suggest that Na+-dependent binding of [H-3]L-aspartate, rather than that of [H-3]D-aspartate, should be further investigated as a possible marker for the glutamatergic/aspartergic synapses in the autoradiographic studies using sections of frozen brain.