Martentoxin, a large-conductance Ca2+-activated K+ channel inhibitor, attenuated TNF-alpha-induced nitric oxide release by human umbilical vein endothelial cells

被引：6

作者：

Wang, Jun ^{[1
]}

Qian, Wenyi ^{[1
]}

Zhu, Qing ^{[1
]}

Chen, Jian ^{[1
]}

Huan, Fei ^{[1
]}

Gao, Rong ^{[1
]}

Xiao, Hang ^{[1
]}

机构：

[1] Nanjing Med Univ, Sch Publ Hlth, Dept Toxicol, Nanjing 210029, Jiangsu, Peoples R China

来源：

JOURNAL OF BIOMEDICAL RESEARCH | 2013年 / 27卷 / 05期

基金：

美国国家科学基金会;

关键词：

martentoxin; Buthus martensii Karsch; nitric oxide; human umbilical vein endothelial cells (HUVECs);

D O I：

10.7555/JBR.27.20120080

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Martentoxin, a 4,046 Da polypeptide toxin purified from the venom of the scorpion Buthus martensii Karsch, has been demonstrated to block large-conductance Ca2+-activated K+ (BKCa) channels; however, its biological roles are still largely unknown. In the present study, we investigated the pharmacological effects of martentoxin on regulating the production of nitric oxide induced by TNF-alpha in human umbilical vein endothelial cells (HU-VECs). We found that, 1, 10 and 100 mu mol/L martentoxin decreased nitric oxide production by HUVECs exposed to 10 ng/mL TNF for 6, 12 and 24 hours. We further demonstrated that martentoxin inhibited the activity of iNOS and retarded the down-regulation of eNOS mRNA induced by TNF-alpha. Therefore, martentoxin could be a potential therapeutic agent for vascular diseases.

引用

页码：386 / 393

页数：8

共 29 条

[1] iNOS-mediated nitric oxide production and its regulation [J].

Aktan, F .

LIFE SCIENCES, 2004, 75 (06) :639-653

[2]

AlMohanna F, 1997, AM J PATHOL, V151, P111

[3]

BUSSE R, 1991, BASIC RES CARDIOL, V86, P7

[4] Purification, characterization of two peptides from Buthus martensi Karch [J].

Cao, ZY ;

Shen, WQ ;

Pan, YP ;

Xiao, X ;

Liu, XM ;

Wang, XL ;

Liang, XT ;

Yu, DQ .

JOURNAL OF PEPTIDE RESEARCH, 2003, 62 (06) :252-259

[5] Neuronal nitric oxide synthase regulates endothelial inflammation [J].

Chakrabarti, Subhadeep ;

Chan, Carmen K. ;

Jiang, Yanyan ;

Davidge, Sandra T. .

JOURNAL OF LEUKOCYTE BIOLOGY, 2012, 91 (06) :947-956

[6] TNF-α contributes to endothelial dysfunction by upregulating arginase in ischemia/reperfusion injury [J].

Gao, Xue ;

Xu, Xiangbin ;

Belmadani, Souad ;

Park, Yoonjung ;

Tang, Zhonghua ;

Feldman, Arthur M. ;

Chilian, William M. ;

Zhang, Cuihua .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2007, 27 (06) :1269-1275

[7] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

[8] Nitric oxide attenuates vascular endothelial cadherin-mediated vascular integrity in human chronic inflammation [J].

Hama, S. ;

Takeichi, O. ;

Fujisaki, K. ;

Tanabe, N. ;

Maeno, M. ;

Ochiai, K. .

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2008, 154 (03) :384-390

[9]

JAFFE EA, 1987, J BIOL CHEM, V262, P8557

[10] Effect of Buthus martensi Karsch on aromatase activity and cytokine-inducded NOS and NO production in osteoblasts and leukaemic cell line FLG 29.1 [J].

Jin, Un-Ho ;

Kim, Kap-Sung ;

Park, Su-Yeon ;

Chung, Kang-Hyun ;

Kim, Dong-Soo ;

Chang, Young-Chae ;

Kim, Cheorl-Ho .

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2006, 28 (02) :241-258

← 1 2 3 →