The sub-iron-iron flux ratio in cosmic rays at an atmospheric depth of 9.8 g cm-2 has been estimated using a balloon-borne CR-39 (HCB) stack launched from Alice Springs for 32 hours. The recovered and chemically etched plates were analysed optically and the measured etch pit diameters yielded the sub-iron-iron flux ratio at the flight altitude. The sub-iron-iron flux ratio has been corrected for the top of the atmosphere by considering the loss of heavy ions due to nuclear interaction and fragmentation. The present result has been compared with the result expected from the source composition derived by Protheroe et al. as well as other authors.