MEMBRANE-VESICLES FROM BROWN ADIPOSE-TISSUE - A TOOL FOR THE STUDY OF AMINO-ACID-TRANSPORT - THE CASE OF L-ALANINE

被引：0
|
作者：
RODRIGUEZMARTIN, A [1 ]
BEL, N [1 ]
REMESAR, X [1 ]
机构：
[1] UNIV BARCELONA,DEPT BIOQUIM & FISIOL,UNITAT BIOQUIM & BIOL MOLEC B,E-08071 BARCELONA,SPAIN
来源：
BIOSCIENCE REPORTS | 1992年 / 12卷 / 02期
关键词：
ALANINE; BROWN ADIPOSE MEMBRANE VESICLES; TRANSPORT;
D O I：
10.1007/BF02351216
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A density gradient method is used to isolate membrane vesicles from brown adipose tissue. These respond to changes in osmolarity and show the classical overshoot pattern when L-alanine uptake is assayed. Transport is shown to be effected by two components: a linear (K(d) = 0.498 min-1) and Na+-dependent saturable component (K(m) = 2.3 mM) and a V(max) = 19.9 pmol/mu-g protein . min). This pattern is similar to that shown by cells isolated from brown adipose tissue.
引用
收藏
页码：115 / 122
页数：8
相关论文
共 50 条
12345