TRANSFORMATION OF ASPERGILLUS-TERREUS WITH THE HYGROMYCIN-B RESISTANCE MARKER FROM ESCHERICHIA-COLI

被引：0
|
作者：
VENTURA, L [1 ]
RAMON, D [1 ]
机构：
[1] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,UNIDAD BIOINGN ALIMENTOS,JAIME ROIG 11,E-46010 VALENCIA,SPAIN
来源：
FEMS MICROBIOLOGY LETTERS | 1991年 / 82卷 / 02期
关键词：
ASPERGILLUS-TERREUS; CHROMOSOMAL INTEGRATION; ESCHERICHIA-COLI GENE; GENETIC TRANSFORMATION; HYGROMYCIN-B RESISTANCE; PLASMID;
D O I：
10.1111/j.1574-6968.1991.tb04863.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aspergillus terreus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of Aspergillus nidulans regulatory sequences. Southern hybridization of transformants indicated that in most of the cases the vector DNA was integrated into the recipient chromosome in the form of tandem arrays. Transformants were mitotically stable in both selective and non-selective medium and retained their capacity to produce xylanase or glucoamylase activities.
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页码：189 / 193
页数：5
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