IMMOBILIZATION OF CATALASE ON MEMBRANES OF POLY(ETHYLENE)-G-CO-ACRYLIC ACID AND POLY(TETRAFLUOROETHYLENE)-G-CO-ACRYLIC ACID AND THEIR APPLICATION IN HYDROGEN-PEROXIDE ELECTROCHEMICAL SENSORS

The immobilization of catalase on grafted membranes of poly(ethylene)-g-co-acrylic acid and poly(tetrafluoroethylene)-g-co-acrylic acid and their application in hydrogen peroxide electrochemical sensors is described. The introduction of carboxylic acid groups onto a hydrophobic support provides a good environment for subsequent enzyme immobilization. This single membrane, hydrogen peroxide sensor showed significant improvement with respect to the double membrane versions. The response is very rapid, the linear range being from 10-mu-M up to 6 mM, with a detection limit of 4.7-mu-M, and a lifetime of more than 4 months.