CHANGES IN ASCORBATE, GLUTATHIONE, AND RELATED ENZYME-ACTIVITIES DURING THIDIAZURON-INDUCED BUD BREAK OF APPLE

被引：2
作者：
WANG, SY [1 ]
JIAO, HJ [1 ]
FAUST, M [1 ]
机构：
[1] GUANGXI AGR COLL,DEPT HORT,NANNING,PEOPLES R CHINA
来源：
PHYSIOLOGIA PLANTARUM | 1991年 / 82卷 / 02期
关键词：
APPLE BUDS; ASCORBATE FREE RADICAL REDUCTASE; ASCORBATE PEROXIDASE; ASCORBIC ACID; DEHYDROASCORBATE REDUCTASE; DEHYDROASCORBIC ACID; DORMANCY BREAKING; GLUTATHIONE; GLUTATHIONE REDUCTASE; MALUS-DOMESTICA; NON-GLUTATHIONE THIOL; TOTAL NONPROTEIN THIOL;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11), dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.
引用
收藏
页码：231 / 236
页数：6
相关论文
共 25 条
  • [1] A RAPID AND SENSITIVE METHOD FOR THE DETERMINATION OF ASCORBIC-ACID USING 4,7-DIPHENYL-1,10-PHENANTHROLINE
    ARAKAWA, N
    TSUTSUMI, K
    SANCEDA, NG
    KURATA, T
    INAGAKI, C
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (05): : 1289 - 1290
  • [2] ASCORBATE FREE-RADICAL REDUCTASE, A KEY ENZYME OF THE ASCORBIC-ACID SYSTEM
    ARRIGONI, O
    DIPIERRO, S
    BORRACCINO, G
    [J]. FEBS LETTERS, 1981, 125 (02): : 242 - 244
  • [3] INTERACTION OF ASCORBATE FREE-RADICAL REDUCTASE WITH SULFHYDRYL-REAGENTS
    BORRACCINO, G
    DIPIERRO, S
    ARRIGONI, O
    [J]. PHYTOCHEMISTRY, 1989, 28 (03) : 715 - 717
  • [4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [5] PURIFICATION, PROPERTIES, AND DISTRIBUTION OF ASCORBATE PEROXIDASE IN LEGUME ROOT-NODULES
    DALTON, DA
    HANUS, FJ
    RUSSELL, SA
    EVANS, HJ
    [J]. PLANT PHYSIOLOGY, 1987, 83 (04) : 789 - 794
  • [6] ENZYMATIC-REACTIONS OF ASCORBATE AND GLUTATHIONE THAT PREVENT PEROXIDE DAMAGE IN SOYBEAN ROOT-NODULES
    DALTON, DA
    RUSSELL, SA
    HANUS, FJ
    PASCOE, GA
    EVANS, HJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) : 3811 - 3815
  • [7] TISSUE SULFHYDRYL GROUPS
    ELLMAN, GL
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) : 70 - 77
  • [8] PRESENCE OF GLUTATHIONE AND GLUTATHIONE REDUCTASE IN CHLOROPLASTS - PROPOSED ROLE IN ASCORBIC-ACID METABOLISM
    FOYER, CH
    HALLIWELL, B
    [J]. PLANTA, 1976, 133 (01) : 21 - 25
  • [9] FRIDOVICH I, 1986, ADV ENZYMOL RAMB, V58, P61
  • [10] Guy C. L., 1982, Plant cold hardiness and freezing stress. Mechanisms and crop implications. Volume 2, P169
123