SEQUENCE SPECIFICITY OF THE CORE-BINDING FACTOR

被引：96

作者：

MELNIKOVA, IN ^{[1
]}

CRUTE, BE ^{[1
]}

WANG, SW ^{[1
]}

SPECK, NA ^{[1
]}

机构：

[1] DARTMOUTH COLL,DEPT BIOCHEM,HANOVER,NH 03755

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 04期

关键词：

D O I：

10.1128/JVI.67.4.2408-2411.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.

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页码：2408 / 2411

页数：4

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