COMPARISON OF AN EBV TRANSFORMED-CELL LINE AND AN EBV HYBRIDOMA CELL-LINE PRODUCING THE SAME HUMAN ANTI-HBS MONOCLONAL-ANTIBODY

被引：3

作者：

SAADU, A

WALKER, H

LOCNISKAR, M

BIDWELL, D

HOWARD, C

MCADAM, KPWJ

VOLLER, A

机构：

[1] UNIV COLL HOSP LONDON,DEPT HAEMATOL,WC1 LONDON,ENGLAND

[2] ZOOL SOC LONDON,INST ZOOL,LONDON NW1 4RY,ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 37卷 / 01期

基金：

英国惠康基金;

关键词：

EBV TRANSFORMED CELL LINE; EBV HYBRIDOMA; HUMAN ANTI-HBS MONOCLONAL ANTIBODY;

D O I：

10.1016/0166-0934(92)90016-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A stable human-mouse heterohybridoma secreting human anti-HBs monoclonal antibody in continuous culture for 12 months was generated. It grew faster than the parent EBV transformed lymphoblastoid cell line (LCL) but produced the same level of specific antibody. The LCL was positive for the Epstein-Barr Virus Nuclear Antigen (EBNA), human CD 23 and contained a diploid number of human chromosomes. The heterohybridoma was negative for EBNA, CD 23 and mouse Ly-1 mouse, despite retaining a full complement of diploid mouse chromosomes and a limited number of human chromosomes.

引用

页码：1 / 12

页数：12

共 25 条

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