COMPLETE NUCLEOTIDE-SEQUENCE OF THE HIGH-MOLECULAR-WEIGHT HUMAN IGF-I MESSENGER-RNA

被引：59

作者：

STEENBERGH, PH

KOONENREEMST, AMCB

CLEUTJENS, CBJM

SUSSENBACH, JS

机构：

[1] Laboratory for Physiological Chemistry, State University of Utrecht Vondellaan 24A

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91593-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

IGF-I gene expression in mammals typically results in multiple mRNA species ranging in size between 0.7 and 7.6 kb. The smaller mRNA species have largely been characterized by the analysis of nearly full-length cDNAs. This report describes the first complete sequence of the prominent high molecular weight (7.6 kb) IGF-I mRNA species. Isolation and nucleotide sequence analysis of cDNA clones from human adult liver and uterus leiomyoma cDNA libraries resulted in a 7236 bp long sequence followed by a poly(A) tail. The sequence data, in combination with structural analysis of the human IGF-I gene, show that the 7.6 kb human IGF-I mRNA contains 6611 bp of untranslated 3′ terminal sequence derived from a single exon. Alternate employment of two polyadenylation signals within the sequence transcribed from this exon generates two mRNAs of 1.1 and 7.6 kb. © 1991.

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页码：507 / 514

页数：8

相关论文

共 25 条

[1] SEQUENCES OF LIVER CDNAS ENCODING 2 DIFFERENT MOUSE INSULIN-LIKE GROWTH FACTOR-I PRECURSORS [J].

BELL, GI ;

STEMPIEN, MM ;

FONG, NM ;

RALL, LB .

NUCLEIC ACIDS RESEARCH, 1986, 14 (20) :7873-7882

[2] NUCLEOTIDE-SEQUENCE OF A GENOMIC FRAGMENT OF THE RAT IGF-I GENE SPANNING AN ALTERNATE 5' NON-CODING EXON [J].

BUCCI, C ;

MALLUCCI, P ;

ROBERTS, CT ;

FRUNZIO, R ;

BRUNI, CB .

NUCLEIC ACIDS RESEARCH, 1989, 17 (09) :3596-3596

[3] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[4] INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II - PEPTIDE, MESSENGER RIBONUCLEIC-ACID AND GENE STRUCTURES, SERUM, AND TISSUE CONCENTRATIONS [J].

DAUGHADAY, WH ;

ROTWEIN, P .

ENDOCRINE REVIEWS, 1989, 10 (01) :68-91

[5] ORGANIZATION OF THE HUMAN GENES FOR INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II [J].

DEPAGTERHOLTHUIZEN, P ;

VANSCHAIK, FMA ;

VERDUIJN, GM ;

VANOMMEN, GJB ;

BOUMA, BN ;

JANSEN, M ;

SUSSENBACH, JS .

FEBS LETTERS, 1986, 195 (1-2) :179-184

[6] TISSUE CONCENTRATIONS OF SOMATOMEDIN-C - FURTHER EVIDENCE FOR MULTIPLE SITES OF SYNTHESIS AND PARACRINE OR AUTOCRINE MECHANISMS OF ACTION [J].

DERCOLE, AJ ;

STILES, AD ;

UNDERWOOD, LE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (03) :935-939

[7] NUCLEOTIDE-SEQUENCE OF THE BOVINE INSULIN-LIKE GROWTH FACTOR-I (IGF-1) AND ITS IGF-1A PRECURSOR [J].

FOTSIS, T ;

MURPHY, C ;

GANNON, F .

NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :676-676

[8]

GLOUDEMANS T, 1990, CANCER RES, V50, P6689

[9] COORDINATE DEVELOPMENTAL REGULATION OF HIGH AND LOW-MOLECULAR-WEIGHT MESSENGER-RNAS FOR RAT INSULIN-LIKE GROWTH FACTOR-II [J].

GRAHAM, DE ;

RECHLER, MM ;

BROWN, AL ;

FRUNZIO, R ;

ROMANUS, JA ;

BRUNI, CB ;

WHITFIELD, HJ ;

NISSLEY, SP ;

SEELIG, S ;

BERRY, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4519-4523

[10]

HELPER JE, 1990, ENDOCRINOLOGY, V127, P1550