CLONING AND SEQUENCE-ANALYSIS OF THE PHENYLALANYL-TRANSFER RNA-SYNTHETASE GENES (PHEST) FROM THERMUS-THERMOPHILUS

被引：18
|
作者：
KELLER, B [1 ]
KAST, P [1 ]
HENNECKE, H [1 ]
机构：
[1] SWISS FED INST TECHNOL, INST MIKROBIOL, SCHMELZBERGSTR 7, CH-8092 ZURICH, SWITZERLAND
来源：
FEBS LETTERS | 1992年 / 301卷 / 01期
关键词：
CLASS-II AMINOACYL-TRANSFER RNA SYNTHETASE; GENE CLONING; NUCLEOTIDE SEQUENCE; PHENYLALANYL-TRANSFER RNA SYNTHETASE; PHES AND PHET GENE;
D O I：
10.1016/0014-5793(92)80215-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
While crystals suitable for X-ray diffraction analyses are available of phenylalanyl-tRNA synthetase (PheRS) from the thermophilic bacterium Thermus thermophilus, neither the primary structure of its constituent alpha and beta-subunits nor the nucleotide sequence of the corresponding pheS and pheT genes were known. Using specific oligonucleotides of conserved pheS regions that were adapted to the T thermophilus codon usage, we identified, cloned and subsequently sequenced the pheST genes of this bacterium. The sequences reported here will greatly aid in the three-dimensional structure determination of T thermophilus PheRS, a heterotetrameric (alpha-2-beta-2), class II aminoacyl-tRNA synthetase.
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页码：83 / 88
页数：6
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