CLONING AND SEQUENCE-ANALYSIS OF THE PHENYLALANYL-TRANSFER RNA-SYNTHETASE GENES (PHEST) FROM THERMUS-THERMOPHILUS

被引：18

作者：

KELLER, B ^{[1
]}

KAST, P ^{[1
]}

HENNECKE, H ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL, INST MIKROBIOL, SCHMELZBERGSTR 7, CH-8092 ZURICH, SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 301卷 / 01期

关键词：

CLASS-II AMINOACYL-TRANSFER RNA SYNTHETASE; GENE CLONING; NUCLEOTIDE SEQUENCE; PHENYLALANYL-TRANSFER RNA SYNTHETASE; PHES AND PHET GENE;

D O I：

10.1016/0014-5793(92)80215-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

While crystals suitable for X-ray diffraction analyses are available of phenylalanyl-tRNA synthetase (PheRS) from the thermophilic bacterium Thermus thermophilus, neither the primary structure of its constituent alpha and beta-subunits nor the nucleotide sequence of the corresponding pheS and pheT genes were known. Using specific oligonucleotides of conserved pheS regions that were adapted to the T thermophilus codon usage, we identified, cloned and subsequently sequenced the pheST genes of this bacterium. The sequences reported here will greatly aid in the three-dimensional structure determination of T thermophilus PheRS, a heterotetrameric (alpha-2-beta-2), class II aminoacyl-tRNA synthetase.

引用

页码：83 / 88

页数：6

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