DEMONSTRATION OF A NEW MOUSE MAMMARY-TUMOR VIRUS LOCUS IN THE GENOME OF THE MAMMARY-TUMOR PRONE STRAIN SHN MOUSE

被引：4

作者：

KOIZUMI, A

TSUKADA, M

WADA, Y

KAMIYAMA, S

机构：

[1] Department of Hygiene, Akita University School of Medicine, Akita

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91950-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Until this report, there have been no detailed analyses of the mouse mammary tumor virus (MMTV) loci in the mammary tumor prone strain SHN. Using a probe, which hybridizes to the env sequence of MMTV, Southern blotting of genomic DNA from the brain after digestion with EcoRI revealed 5 endogenous proviruses: Mtv-1 (4.5kb), Mtv-2 (11kb), Mtv-8 (6.7kb), Mtv-17 (8.3kb) and a newly-found 6.5-kb fragment. Fl-hybrid mice ( C3H He female × SHN male) also possessed the 6.5-kb fragment. Thus, we conclude that the 6.5-kb fragment is unique to SHN mice. Genomic DNA from mammary tumors of SHN mice showed MMTV insertions, suggesting that activation of an oncogene(s) occurred in this strain. © 1990.

引用

页码：336 / 341

页数：6

共 16 条

[1]

BENTVELZEN P, 1968, GENETIC CONTROL VERT

[2] ENDOGENOUS MAMMARY-TUMOR VIRUS-DNA VARIES AMONG WILD MICE AND SEGREGATES DURING INBREEDING [J].

COHEN, JC ;

VARMUS, HE .

NATURE, 1979, 278 (5703) :418-423

[3]

IMAI S, 1983, J NATL CANCER I, V71, P517

[4] CALORIC RESTRICTION PERTURBS THE PITUITARY-OVARIAN AXIS AND INHIBITS MOUSE MAMMARY-TUMOR VIRUS PRODUCTION IN A HIGH-SPONTANEOUS-MAMMARY-TUMOR-INCIDENCE MOUSE STRAIN (C3H/SHN) [J].

KOIZUMI, A ;

MASUDA, H ;

WADA, Y ;

TSUKADA, M ;

KAWAMURA, K ;

KAMIYAMA, S ;

WALFORD, RL .

MECHANISMS OF AGEING AND DEVELOPMENT, 1989, 49 (02) :93-104

[5] A STANDARDIZED NOMENCLATURE FOR ENDOGENOUS MOUSE MAMMARY-TUMOR VIRUSES [J].

KOZAK, C ;

PETERS, G ;

PAULEY, R ;

MORRIS, V ;

MICHALIDES, R ;

DUDLEY, J ;

GREEN, M ;

DAVISSON, M ;

PRAKASH, O ;

VAIDYA, A ;

HILGERS, J ;

VERSTRAETEN, A ;

HYNES, N ;

DIGGELMANN, H ;

PETERSON, D ;

COHEN, JC ;

DICKSON, C ;

SARKAR, N ;

NUSSE, R ;

VARMUS, H ;

CALLAHAN, R .

JOURNAL OF VIROLOGY, 1987, 61 (05) :1651-1654

[6]

Maniatis T., 1982, MOL CLONING

[7] THE INDUCTION OF ADENOMYOSIS IN MICE BY INTRAUTERINE PITUITARY ISOGRAFTS [J].

MORI, T ;

NAGASAWA, H ;

TAKAHASHI, S .

LIFE SCIENCES, 1981, 29 (12) :1277-1282

[8]

Muhlbock O, 1965, Eur J Cancer, V1, P123, DOI 10.1016/0014-2964(65)90003-4

[9]

MURPHY ED, 1968, BIOL LABORATORY MOUS

[10]

NAGASAWA H, 1976, GANN, V67, P913

← 1 2 →