ENZYMATIC-PROPERTIES OF LIPASE AND CHARACTERISTICS PRODUCTION BY LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS

被引：16

作者：

ELSAWAH, MMA ^{[1
]}

SHERIEF, AA ^{[1
]}

BAYOUMY, SM ^{[1
]}

机构：

[1] MANSOURA UNIV,FAC SCI,DEPT BOT,MANSOURA,EGYPT

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1995年 / 67卷 / 04期

关键词：

LACTOBACILLUS DELBRUECKII SUBSP BULGARICUS; LIPASE;

D O I：

10.1007/BF00872935

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Some properties of an extracellular lipase produced by Lactobacillus delbrueckii subsp. bulgaricus were studied. Maximum enzyme activity was found against olive and butter oil as enzyme substrates. Addition of 9% acacia gum, 0.1% Na-deoxycholate and 0.01 M CaCl2 to the enzyme reaction mixture increased-lipase activity from 5.3 to 14.5 (FFA/mg protein/minute) at pH 6.0 and at 40 degrees C. Maximum lipase production was reached in the presence of glucose as a sole source of carbon, wheat bran as nitrogen source, olive oil as a sole lipid source and butyric acid as fatty acid supporting the growth medium. An initial pH value of the culture medium of 6.0 and a temperature of 35 degrees C gave the highest lipolytic activity.

引用

页码：357 / 362

页数：6

共 17 条

[1] FACTORS INFLUENCING THE ACTIVITY OF A HEAT-RESISTANT LIPASE OF PSEUDOMONAS [J].