CYCLIC-AMP INHIBITS C-HA-RAS PROTOONCOGENE EXPRESSION AND DNA-SYNTHESIS IN RAT AORTIC SMOOTH-MUSCLE CELLS

被引：28
|
作者：
SADHU, DN
RAMOS, KS
机构：
[1] Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, Texas
来源：
EXPERIENTIA | 1993年 / 49卷 / 6-7期
关键词：
C-HA-RAS; AORTIC SMOOTH MUSCLE CELLS; CELL CYCLE; CYCLIC AMP; PROLIFERATION;
D O I：
10.1007/BF01955165
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (G0-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibutyryl cyclic AMP (1-100 muM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and prevented progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.
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页码：567 / 570
页数：4
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