ENDOTHELIN SUBTYPE-B RECEPTORS ARE COUPLED TO ADENYLATE-CYCLASE VIA INHIBITORY-G PROTEIN IN CULTURED BOVINE ENDOTHELIAL-CELLS

被引：18

作者：

EGUCHI, S ^{[1
]}

HIRATA, Y ^{[1
]}

MARUMO, F ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV,DEPT INTERNAL MED 2,DIV ENDOCRINE HYPERTENS,1-5-45 YUSHIMA,BUNKYO KU,TOKYO 113,JAPAN

来源：

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | 1993年 / 22卷

关键词：

ENDOTHELIN; ET(B) RECEPTOR; ENDOTHELIAL CELL; ADENYLATE CYCLASE; GI PROTEIN;

D O I：

10.1097/00005344-199322008-00043

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We studied whether endothelin (ET) isopeptides have any effects on adenylate cyclase activity in cultured bovine endothelial cells (ECs). Both ET-1 and ET-3 dose-dependently inhibited cAMP formation stimulated by forskolin and isoproterenol, although the inhibitory effect of ET-1 was less potent than that of ET-3. In contrast, ET-1 and ET-3 almost equipotently inhibited forskolin-stimulated cAMP formation in bovine EC pre-treated with phosphoramidon, a putative ET-1-converting-enzyme inhibitor. These data suggest that endothelial ET(B) receptors are functionally coupled to adenylate cyclase, possibly via G(i) protein.

引用

页码：S161 / S163

页数：3

共 15 条

[1] CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR
ARAI, H
HORI, S
ARAMORI, I
OHKUBO, H
NAKANISHI, S
[J]. NATURE, 1990, 348 (6303) : 730 - 732
[2] ARAMORI I, 1992, J BIOL CHEM, V267, P12468
[3] ENDOTHELIN RECEPTOR SUBTYPES ARE COUPLED TO ADENYLATE-CYCLASE VIA DIFFERENT GUANYL NUCLEOTIDE-BINDING PROTEINS IN VASCULATURE
EGUCHI, S
HIRATA, Y
IMAI, T
MARUMO, F
[J]. ENDOCRINOLOGY, 1993, 132 (02) : 524 - 529
[4] A NOVEL ETA ANTAGONIST (BQ-123) INHIBITS ENDOTHELIN-1-INDUCED PHOSPHOINOSITIDE BREAKDOWN AND DNA-SYNTHESIS IN RAT VASCULAR SMOOTH-MUSCLE CELLS
EGUCHI, S
HIRATA, Y
IHARA, M
YANO, M
MARUMO, F
[J]. FEBS LETTERS, 1992, 302 (03) : 243 - 246
[5] IDENTIFICATION OF G-PROTEIN-COUPLED ENDOTHELIN RECEPTORS IN CULTURED BOVINE ENDOTHELIAL-CELLS
EGUCHI, S
KOZUKA, M
HIROSE, S
ITO, T
HIRATA, Y
MARUMO, F
HAGIWARA, H
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (03) : 1343 - 1346
[6] ENDOTHELIN-3 STIMULATES PRODUCTION OF ENDOTHELIUM-DERIVED NITRIC-OXIDE VIA PHOSPHOINOSITIDE BREAKDOWN
EMORI, T
HIRATA, Y
KANNO, K
OHTA, K
EGUCHI, S
IMAI, T
SHICHIRI, M
MARUMO, F
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (01) : 228 - 235
[7] SPECIFIC RECEPTORS FOR ENDOTHELIN-3 IN CULTURED BOVINE ENDOTHELIAL-CELLS AND ITS CELLULAR MECHANISM OF ACTION
EMORI, T
HIRATA, Y
MARUMO, F
[J]. FEBS LETTERS, 1990, 263 (02) : 261 - 264
[8] AUTOCRINE RECEPTORS FOR ENDOTHELINS IN THE PRIMARY CULTURE OF ENDOTHELIAL-CELLS OF HUMAN UMBILICAL VEIN
FUJITANI, Y
ODA, K
TAKIMOTO, M
INUI, T
OKADA, T
URADE, Y
[J]. FEBS LETTERS, 1992, 298 (01) : 79 - 83
[9] THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES
INOUE, A
YANAGISAWA, M
KIMURA, S
KASUYA, Y
MIYAUCHI, T
GOTO, K
MASAKI, T
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) : 2863 - 2867
[10] MARTIN P, 1991, J NEUROCHEM, V56, P1270

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