USE OF THE RAPD PROCEDURE FOR THE IDENTIFICATION OF TRICHODERMA STRAINS

被引：66

作者：

ZIMAND, G

VALINSKY, L

ELAD, Y

CHET, I

MANULIS, S

机构：

[1] ARO,VOLCANI CTR,DEPT PLANT PATHOL,IL-50250 BET DAGAN,ISRAEL

[2] HEBREW UNIV JERUSALEM,FAC AGR,DEPT PLANT PATHOL & MICROBIOL,IL-76100 REHOVOT,ISRAEL

来源：

MYCOLOGICAL RESEARCH | 1994年 / 98卷

关键词：

D O I：

10.1016/S0953-7562(09)80474-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.

引用

页码：531 / 534

页数：4

共 13 条

[1]

[Anonymous], 1969, MYCOL PAP

[2]

BISSETT J, 1984, CAN J BOT, V62, P9624

[3] SUBSTRATE-DEPENDENT VARIATION IN THE PROTEIN PROFILE AND ANTIGENS OF TRICHODERMA-HARZIANUM [J].