A SIMPLE METHOD FOR QUANTIFYING SPECIFIC MESSENGER-RNAS IN SMALL NUMBERS OF EARLY MOUSE EMBRYOS

被引：43

作者：

GAUDETTE, MF ^{[1
]}

CRAIN, WR ^{[1
]}

机构：

[1] WORCESTER FDN EXPTL BIOL INC, CELL BIOL GRP, 222 MAPLE AVE, SHREWSBURY, MA 01545 USA

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 08期

关键词：

D O I：

10.1093/nar/19.8.1879

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amplification of sequences by the polymerase chain reaction (PCR) has become a powerful tool in the study of gene expression. The technique is, in fact, so powerful that it may detect 'leaky transcription'. Thus, it is now imporant to be able to quantify the transcripts that are amplified to determine whether or not they represent legitimate transcription of target genes. In this paper, we describe a one-step amplification reaction coupled to solution hybridization/RNase protection that is capable of quantitating specific transcripts in total RNA from one to ten preimplantation mouse embryos and is generally applicable to any cloned mRNA sequence.

引用

页码：1879 / 1884

页数：6

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