RAPID PULSED-FIELD CAPILLARY ELECTROPHORETIC SEPARATION OF MEGABASE NUCLEIC-ACIDS

被引：68

作者：

KIM, YS ^{[1
]}

MORRIS, MD ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 05期

关键词：

D O I：

10.1021/ac00101a002

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Pulsed field capillary electrophoresis in ultradilute solutions of sieving polymers has been used to separate nucleic acid fragments as long as 1.6 million base pairs. Hydroxyethyl cellulose solutions are used for separations in the size range 8000-50 000 base pairs. Longer chain nucleic acids are separated in mixed hydroxyethyl cellulose/poly(ethylene oxide) solutions. Separations are rapid (12-13 min).

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页码：784 / 786

页数：3

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