PRIMER-INDUCED IN-SITU HYBRIDIZATION TO PLANT CHROMOSOMES

被引：21

作者：

ABBO, S

MILLER, TE

KING, IP

机构：

[1] Cambridge Laboratory, Norwich NR4 7UJ, Colney lane

来源：

GENOME | 1993年 / 36卷 / 05期

关键词：

PRIMER-INDUCED IN-SITU HYBRIDIZATION; RYE (SECALE-CEREALE L); NUCLEOLUS ORGANIZER REGION;

D O I：

10.1139/g93-107

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primer-induced in situ hybridization of high copy sequences was used successfully on rye mitotic chromosome spreads. Nucleolus organizer sequences were detected in rye, and the pattern obtained was in full agreement with previously reported results by conventional in situ hybridization. The future potential of the primer-induced in situ hybridization technique is briefly discussed.

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页码：815 / 817

页数：3

相关论文

共 17 条

[1] DETECTION OF A 17-KB UNIQUE SEQUENCE (T-DNA) IN PLANT CHROMOSOMES BY INSITU HYBRIDIZATION [J].

AMBROS, PF ;

MATZKE, MA ;

MATZKE, AJM .

CHROMOSOMA, 1986, 94 (01) :11-18

[2] PHYSICAL MAPPING OF THE B-HORDEIN LOCI ON BARLEY CHROMOSOME-5 BY INSITU HYBRIDIZATION [J].

CLARK, M ;

KARP, A ;

ARCHER, S .

GENOME, 1989, 32 (05) :925-929

[3] MAPPING SMALL DNA-SEQUENCES BY FLUORESCENCE INSITU HYBRIDIZATION DIRECTLY ON BANDED METAPHASE CHROMOSOMES [J].

FAN, YS ;

DAVIS, LM ;

SHOWS, TB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6223-6227

[4] CLONING AND CHARACTERIZATION OF RIBOSOMAL-RNA GENES FROM WHEAT AND BARLEY [J].

GERLACH, WL ;

BEDBROOK, JR .

NUCLEIC ACIDS RESEARCH, 1979, 7 (07) :1869-1885

[5]

GOSDEN J, 1993, IN PRESS BIOTECHNIQU

[6] PHYSICAL MAPPING OF A LOW-COPY DNA-SEQUENCE IN RYE (SECALE-CEREALE L) [J].

GUSTAFSON, JP ;

BUTLER, E ;

MCINTYRE, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) :1899-1902

[7]

Heslop-Harrison J. S., 1991, Technique, a Journal of Methods in Cell and Molecular Biology, V3, P109

[8]

HESLOPHARRISON JS, 1991, J CELL SCI, V100, P15

[9] FAST ONE-STEP PROCEDURE FOR THE DETECTION OF NUCLEIC-ACIDS INSITU BY PRIMER-INDUCED SEQUENCE-SPECIFIC LABELING WITH FLUORESCEIN-12-DUTP [J].

KOCH, J ;

MOGENSEN, J ;

PEDERSEN, S ;

FISCHER, H ;

HINDKJAER, J ;

KOLVRAA, S ;

BOLUND, L .

CYTOGENETICS AND CELL GENETICS, 1992, 60 (01) :1-&

[10] AN IMPROVED METHOD FOR CHROMOSOME-SPECIFIC LABELING OF ALPHA-SATELLITE DNA INSITU BY USING DENATURED DOUBLE-STRANDED DNA PROBES AS PRIMERS IN A PRIMED INSITU LABELING (PRINS) PROCEDURE [J].

KOCH, J ;

HINDKJAER, J ;

MOGENSEN, J ;

KOLVRAA, S ;

BOLUND, L .

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING, 1991, 8 (06) :171-178