MICROCELL-MEDIATED TRANSFER OF A SINGLE HUMAN-CHROMOSOME COMPLEMENTS XERODERMA-PIGMENTOSUM GROUP-A FIBROBLASTS

被引：32
作者：
SCHULTZ, RA
SAXON, PJ
GLOVER, TW
FRIEDBERG, EC
机构：
[1] UNIV MICHIGAN,DEPT PEDIAT,ANN ARBOR,MI 48109
[2] UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48109
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1987年 / 84卷 / 12期
关键词：
D O I：
10.1073/pnas.84.12.4176
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Chromosomes from an immortalized aneuploid human fibroblast cell line were randomly tagged with the selectable marker neo by transfection with the plasmid pSV2neo. Somatic cell fusions between transfected human cells and mouse A9 cells generated pools of G418-resistant human-mouse hybrid clones containing various numbers of human chromosomes. Microcell-mediated chromosome transfer from the hybrid pools to xeroderma pigmentosum complementation group A (XP-A) cells in culture and selection for G418-resistant colonies resulted in the identification of XP cells with enhanced resistance to ultraviolet radiation. Screening of subclones from selected pools of human-mouse hybrids facilitated the identification of hybrids containing a single neo-tagged human chromosome. Transfer of this chromosome to XP-A cells (but not to XP-F or XP-C cells) results in ehanced resistance to ultraviolet light and ehanced excision repair capacity. The identification of a single human chromosome that complements the phenotype of XP-A cells in culture provides the potential for genetic mapping of the complementing gene and for its isolation by molecular cloning.
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页码：4176 / 4179
页数：4
相关论文
共 13 条
[1]   INTEGRATION OF A DOMINANT SELECTABLE MARKER INTO HUMAN-CHROMOSOMES AND TRANSFER OF MARKED CHROMOSOMES TO MOUSE CELLS BY MICROCELL FUSION [J].
ATHWAL, RS ;
SMARSH, M ;
SEARLE, BM ;
DEO, SS .
SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (02) :177-187
[2]   ISOLATION AND PARTIAL CHARACTERIZATION OF VIRUS-TRANSFORMED CELL-LINES REPRESENTING THE A, G AND VARIANT COMPLEMENTATION GROUPS OF XERODERMA-PIGMENTOSUM [J].
BARBIS, DP ;
SCHULTZ, RA ;
FRIEDBERG, EC .
MUTATION RESEARCH, 1986, 165 (03) :175-184
[3]   MICROCELL-MEDIATED TRANSFER OF MURINE CHROMOSOMES INTO MOUSE, CHINESE-HAMSTER, AND HUMAN SOMATIC-CELLS [J].
FOURNIER, REK ;
RUDDLE, FH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (01) :319-323
[4]   A GENERAL HIGH-EFFICIENCY PROCEDURE FOR PRODUCTION OF MICROCELL HYBRIDS [J].
FOURNIER, REK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (10) :6349-6353
[5]  
FRIEDBERG EC, 1985, DNA REPAIR, P506
[6]  
GANESAN A, 1982, MANIPULATION EXPRESS, P45
[7]   LINKAGE OF HUMAN APOLIPOPROTEIN-A-I AND APOLIPOPROTEIN-C-III GENES [J].
KARATHANASIS, SK ;
MCPHERSON, J ;
ZANNIS, VI ;
BRESLOW, JL .
NATURE, 1983, 304 (5924) :371-373
[8]   ISOLATION OF UV-RESISTANT REVERTANTS FROM A XERODERMA PIGMENTOSUM COMPLEMENTATION GROUP A-CELL LINE [J].
ROYERPOKORA, B ;
HASELTINE, WA .
NATURE, 1984, 311 (5984) :390-392
[9]   SELECTIVE TRANSFER OF INDIVIDUAL HUMAN-CHROMOSOMES TO RECIPIENT CELLS [J].
SAXON, PJ ;
SRIVATSAN, ES ;
LEIPZIG, GV ;
SAMESHIMA, JH ;
STANBRIDGE, EJ .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (01) :140-146
[10]   STUDIES ON GENE-TRANSFER AND REVERSION TO UV RESISTANCE IN XERODERMA PIGMENTOSUM-CELLS [J].
SCHULTZ, RA ;
BARBIS, DP ;
FRIEDBERG, EC .
SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (06) :617-624
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