PROLACTIN BINDING IN RAT MAMMARY-GLAND DURING PREGNANCY AND LACTATION

被引:70
作者
HOLCOMB, HH [1 ]
COSTLOW, ME [1 ]
BUSCHOW, RA [1 ]
MCGUIRE, WL [1 ]
机构
[1] UNIV TEXAS, CTR HLTH SCI, DEPT MED, SAN ANTONIO, TX 78284 USA
关键词
D O I
10.1016/0304-4165(76)90112-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lactogenic hormones from the placenta and pituitary are primarily responsible for the growth and function of the mammary gland during pregnancy and lactation. The optimal conditions for the measurement of 125I-labeled ovine prolactin binding to mammary gland slices of pregnant and lactating rats are described. Prolactin binding is saturable (Kd about 2.36 .cntdot. 10-9 M), hormone specific and destroyed by proteases. The hormonal environments of pregnancy and lactation dramatically influence the availability and measurement of prolactin binding sites. Whereas binding consistently appears to be low in mammary glands removed from rats during pregnancy, binding levels rise 7-8-fold shortly after birth and remain high during the 22 days of lactation. The removal of the ovaries and gravid uteri at specific times during pregnancy results in a prompt 3-6-fold increase in prolactin binding. Elevated levels in potential prolactin binding capacity appear in mammary tissue coincident with the reported rise in serum rat placental lactogen between the 8th and 11th days. High levels of this lactogenic hormone promote the appearance of prolactin binding sites during pregnancy and mask the sites such that they are not available for measurement in vitro.
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收藏
页码:104 / 112
页数:9
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