SUBDOMAIN STRUCTURE OF LIPID-BOUND ANNEXIN-V RESOLVED BY ELECTRON IMAGE-ANALYSIS

被引:128
作者
MOSSER, G [1 ]
RAVANAT, C [1 ]
FREYSSINET, JM [1 ]
BRISSON, A [1 ]
机构
[1] CTR REG TRANSFUS SANGUINE,INSERM,U311,F-67085 STRASBOURG,FRANCE
关键词
D O I
10.1016/0022-2836(91)90538-H
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two-dimensional crystals of annexin-V bound to lipid layers containing dioleoyl-phosphatidylserine have been obtained in the presence of Ca2+. The crystals diffract to 20 Å resolution and have the symmetry of the plane group p3 (unit cell dimensions: a = b = 94 A ̊, γ = 120 °). Electron image analysis revealed that the crystals are composed of trimers of annexin-V forming triskelion-like motifs. Each annexin-V molecule has a characteristic elongated shape, about 65 Å by 20 Å, when observed perpendicularly to the crystal plane. It is composed of two staggered domains of similar size, about 40 Å by 20 Å. Both domains are made of two sub-domains. The present data suggest that the four resolved sub-domains represent the folding units corresponding to the four 70 amino acid repeating segments characteristic of all annexins. © 1991.
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页码:241 / 245
页数:5
相关论文
共 29 条
[1]   3-DIMENSIONAL STRUCTURE DETERMINATION BY ELECTRON-MICROSCOPY OF TWO-DIMENSIONAL CRYSTALS [J].
AMOS, LA ;
HENDERSON, R ;
UNWIN, PNT .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1983, 39 (03) :183-231
[2]  
ANDREE HAM, 1990, J BIOL CHEM, V265, P4923
[3]   CHARACTERIZATION OF CA-2+-DEPENDENT PHOSPHOLIPID BINDING, VESICLE AGGREGATION AND MEMBRANE-FUSION BY ANNEXINS [J].
BLACKWOOD, RA ;
ERNST, JD .
BIOCHEMICAL JOURNAL, 1990, 266 (01) :195-200
[4]   ISOLATION AND CHARACTERIZATION OF 2 MEMBRANE-ASSOCIATED PLACENTAL TISSUE PROTEINS [J].
BOHN, H ;
KRAUS, W ;
WINCKLER, W .
ARCHIVES OF GYNECOLOGY, 1985, 236 (04) :225-233
[5]   DIVERSITY IN THE LIPOCORTIN CALPACTIN FAMILY [J].
CROMPTON, MR ;
MOSS, SE ;
CRUMPTON, MJ .
CELL, 1988, 55 (01) :1-3
[6]  
CRUJPTON MJ, 1990, NATURE, V345, P212
[7]  
DAVIDSON FF, 1987, J BIOL CHEM, V262, P1698
[8]  
FLAHERTY MJ, 1990, J LAB CLIN MED, V115, P174
[9]   PRIMARY STRUCTURE OF HUMAN PLACENTAL ANTICOAGULANT PROTEIN [J].
FUNAKOSHI, T ;
HENDRICKSON, LE ;
MCMULLEN, BA ;
FUJIKAWA, K .
BIOCHEMISTRY, 1987, 26 (25) :8087-8092
[10]   HUMAN PLACENTAL ANTICOAGULANT PROTEIN - ISOLATION AND CHARACTERIZATION [J].
FUNAKOSHI, T ;
HEIMARK, RL ;
HENDRICKSON, LE ;
MCMULLEN, BA ;
FUJIKAWA, K .
BIOCHEMISTRY, 1987, 26 (17) :5572-5578