PROTEIN-KINASE-C IN RAT-BRAIN SYNAPTOSOMES - BETA-II-SUBSPECIES AS A MAJOR ISOFORM ASSOCIATED WITH MEMBRANE-SKELETON ELEMENTS

被引：23

作者：

TANAKA, S ^{[1
]}

TOMINAGA, M ^{[1
]}

YASUDA, I ^{[1
]}

KISHIMOTO, A ^{[1
]}

NISHIZUKA, Y ^{[1
]}

机构：

[1] KOBE UNIV, BIOSIGNAL RES CTR, KOBE 657, JAPAN

来源：

FEBS LETTERS | 1991年 / 294卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

PROTEIN KINASE-C; SYNAPTOSOME; MEMBRANE-SKELETON; GROWTH-ASSOCIATED PROTEIN-43; RAT BRAIN;

D O I：

10.1016/0014-5793(91)81445-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A small fraction (approximately 5%) of protein kinase C (PKC) in the adult rat brain synaptosomes is tightly associated with Triton X-100-insoluble components (most likely membrane-skeleton elements), and is solubilized only after denaturation with sodium dodecyl sulfate. The kinase domain of this PKC can be released as a soluble form after limited proteolysis with calpain, whereas the regulatory domain which binds phorbol ester remains insoluble. The PKC in this fraction was identified as the beta-II-subspecies or its related molecule. Presumably, this enzyme subspecies is responsible for the phosphorylation of a major PKC substrate protein, growth-associated protein-43, which is located in nerve endings as well as in growth cones in association with the membrane-skeleton elements.

引用

页码：267 / 270

页数：4

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