ENZYME BIOSENSOR FOR UREA BASED ON A NOVEL PH BULK OPTODE MEMBRANE

被引：53

作者：

KONCKI, R ^{[1
]}

MOHR, GJ ^{[1
]}

WOLFBEIS, OS ^{[1
]}

机构：

[1] KARL FRANZENS UNIV GRAZ,INST ORGAN CHEM,DIV ANALYT,A-8010 GRAZ,AUSTRIA

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 08期

基金：

美国国家科学基金会;

关键词：

BIOSENSOR; PH OPTODE; ENZYME OPTODE; ENZYME IMMOBILIZATION; UREA SENSOR;

D O I：

10.1016/0956-5663(95)96955-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new, absorbance-based enzymatic biosensor membrane for determination of urea is described. A lipophilic, fully LED- and diode laser-compatible pH sensitive dye was incorporated into a plasticized, carboxylated poly(vinyl chloride) membrane and served as the optical transducer of the sensor. Urease was covalently linked to the surface of the pH bulk optode membrane to farm a very thin cover. The resulting biosensor membrane allows rapid determination of urea over the 0.3 to 100 mM range. The reproducibility, stability, and effects of pH and buffer concentration on the response of sensor are reported. The preparation of the pH transducer and the immobilization of the enzyme are simple and may easily be adopted to other biosensor types.

引用

页码：653 / 659

页数：7

共 21 条

[1] UREA SENSORS BASED ON PVC MEMBRANE PH ELECTRODE [J].