PROTEIN INSERTION INTO THE ENDOPLASMIC-RETICULUM OF PERMEABILIZED CELLS

被引：7
|
作者：
JADOT, M [1 ]
HOFMANN, MW [1 ]
GRAF, R [1 ]
QUADER, H [1 ]
机构：
[1] UNIV HEIDELBERG,ZMBH,ZENTRUM MOLEK BIOL,D-69120 HEIDELBERG,GERMANY
来源：
FEBS LETTERS | 1995年 / 371卷 / 02期
关键词：
CELL PERMEABILIZATION; SITE-SPECIFIC PHOTO-CROSS-LINKING; ENDOPLASMIC RETICULUM; PROTEIN TRANSLOCATION;
D O I：
10.1016/0014-5793(95)00881-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells, By site-specific photo-crosslinking we show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes, This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.
引用
收藏
页码：145 / 148
页数：4
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