EVIDENCE THAT THE MECHANISM OF PRENATAL GERM-CELL DEATH IN THE MOUSE IS APOPTOSIS

被引：173

作者：

COUCOUVANIS, EC ^{[1
]}

SHERWOOD, SW ^{[1
]}

CARSWELLCRUMPTON, C ^{[1
]}

SPACK, EG ^{[1
]}

JONES, PP ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT BIOL SCI,STANFORD,CA 94305

来源：

EXPERIMENTAL CELL RESEARCH | 1993年 / 209卷 / 02期

关键词：

D O I：

10.1006/excr.1993.1307

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Using fluorescence-activated cell sorting combined with fluorescence microscopy the mechanism of embryonic germ cell death in the mouse has been shown to be apoptosis. Primordial germ cells (PGCs) from embryos at specific developmental stages have been analyzed, and cells with apoptotic morphology have been isolated by cell sorting. In the female, apoptotic oogonia at Day 13 and apoptotic oocytes at Days 15 and 17 were found. In the male, apoptotic cells were seen on Day 13 through Day 17. Apoptotic germ cells were not detected at Day 12 (combined male and female PGCs). Examination of sorted cells by fluorescence microscopy and by light microscopic analysis after alkaline phosphatase staining confirmed that the cells are apoptotic germ cells. Electron microscopy further confirmed that cells showing the morphological characteristics of apoptosis are present. © 1993 Academic Press, Inc.

引用

页码：238 / 247

页数：10

共 33 条

[1]

ADAMSON ED, 1987, DEVELOPMENT, V99, P449

[2] PATTERNS OF RNA-SYNTHESIS IN EARLY MEIOTIC PROPHASE OOCYTES FROM FETAL MOUSE OVARIES [J].