HEMOLYSIS OF RABBIT ERYTHROCYTES IN THE PRESENCE OF COPPER IONS - INHIBITION BY ALBUMIN AND CERULOPLASMIN

被引:6
作者
CAFFREY, JM [1 ]
SMITH, HA [1 ]
SCHMITZ, JC [1 ]
MERCHANT, A [1 ]
FRIEDEN, E [1 ]
机构
[1] FLORIDA STATE UNIV,DEPT CHEM,TALLAHASSEE,FL 32306
关键词
albumin; ceruloplasmin; ceruloplasmin-Cu(II) complexes; Cu(II) induced hemolysis; Cu(II) uptake; hemolysis inhibition by ceruloplasmin; MetHb formation; Red blood cells;
D O I
10.1007/BF02990259
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hemolysis of red blood cells (RBC) induced by Cu(II) is modified by ceruloplasmin (Cp) and albumin. The time course of hemolysis for rabbit RBC by Cu(II) consisted of two parts, an induction period followed by a catastrophic lysis period. The induction period decreased and the lysis rate increased with increasing Cu(II) concentration. Cp or albumin, modified Cu(II) induced hemolysis, by increasing the duration of the induction period and decreasing the overall rate of hemolysis of RBC. The catastrophic lysis period coincided with a sharp increase in the formation of metHb within the cell and in a rapid uptake of Cu(II). The presence of Cp led to an increase in the induction period prior to the rapid increase in metHb formation and in Cu(II) uptake. Porcine Cp was prepared with either two or three nonprosthetic copper binding sties (sites where Cu(II) is easily removed by passing over Chelex-100). Cp with three nonprosthetic binding sites gave more protection than Cp with two. Likewise, albumin can be prepared with three and five nonprosthetic copper binding sites. The albumin with five sites gave more protection than the albumin with three sites. © 1990 Humana Press Inc.
引用
收藏
页码:11 / 19
页数:9
相关论文
共 15 条
[1]  
ASHMUN RA, 1984, RED CELL, P309
[2]   CERULOPLASMIN RECEPTORS OF ERYTHROCYTES [J].
BARNES, G ;
FRIEDEN, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 125 (01) :157-162
[3]  
BARNES G, 1985, 5TH P INT S TRAC EL, P143
[4]   EQUATIONS FOR SPECTROPHOTOMETRIC ANALYSIS OF HEMOGLOBIN MIXTURES [J].
BENESCH, RE ;
BENESCH, R ;
YUNG, S .
ANALYTICAL BIOCHEMISTRY, 1973, 55 (01) :245-248
[5]   THE EFFECT OF COPPER-ION ON GLUTATHIONE AND HEMOLYSIS IN RABBIT ERYTHROCYTES [J].
CAFFREY, JM ;
DASMAHAPATRA, A ;
SMITH, HA ;
HEDE, K ;
FRIEDEN, E .
BIOLOGICAL TRACE ELEMENT RESEARCH, 1986, 11 :19-26
[6]  
GOLDSTEIN IM, 1979, J BIOL CHEM, V254, P4040
[9]  
HOCHSTEIN P, 1978, RED CELL, P669
[10]   THE PROTECTIVE ACTION OF CERULOPLASMIN ON COPPER-ION STIMULATED LYSIS OF RAT ERYTHROCYTES [J].
LOVSTAD, RA .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1982, 14 (07) :585-589