Distinctive effects of CD34- and CD133-specific antibody-coated stents on re-endothelialization and in-stent restenosis at the early phase of vascular injury

被引：41

作者：

Wu, Xue ^{[1
]}

Yin, Tieying ^{[1
]}

Tian, Jie ^{[1
]}

Tang, Chaojun ^{[1
]}

Huang, Junli ^{[1
]}

Zhao, Yinping ^{[1
]}

Zhang, Xiaojuan ^{[1
]}

Deng, Xiaoyan ^{[2
]}

Fan, Yubo ^{[2
]}

Yu, Donghong ^{[3
]}

Wang, Guixue ^{[1
]}

机构：

[1] Chongqing Univ, Minist Educ, Bioengn Coll, Chongqing Engn Lab Vasc Implants, 174 Shazhengjie, Chongqing 400044, Peoples R China

[2] Beihang Univ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China

[3] Aalborg Univ, Dept Biotechnol Chem & Environm Engn, DK-9220 Aalborg, Denmark

来源：

REGENERATIVE BIOMATERIALS | 2015年 / 2卷 / 02期

基金：

中国国家自然科学基金;

关键词：

stent; CD133; CD34; endothelial progenitor cells; re-endothelialization;

D O I：

10.1093/rb/rbv007

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

It is not clear what effects of CD34- and CD133-specific antibody-coated stents have on re-endothelialization and in-stent restenosis (ISR) at the early phase of vascular injury. This study aims at determining the capabilities of different coatings on stents (e.g. gelatin, anti-CD133 and anti-CD34 antibodies) to promote adhesion and proliferation of endothelial progenitor cells (EPCs). The in vitro study revealed that the adhesion force enabled the EPCs coated on glass slides to withstand flow-induced shear stress, so that allowing for the growth of the cells on the slides for 48 h. The in vivo experiment using a rabbit model in which the coated stents with different substrates were implanted showed that anti-CD34 and anti-CD133 antibody-coated stents markedly reduced the intima area and restenosis than bare mental stents (BMS) and gelatin-coated stents. Compared with the anti-CD34 antibody-coated stents, the time of cells adhesion was longer and earlier present in the anti-CD133 antibody-coated stents and anti-CD133 antibody-coated stents have superiority in re-endothelialization and inhibition of ISR. In conclusion, this study demonstrated that anti-CD133 antibody as a stent coating for capturing EPCs is better than anti-CD34 antibody in promoting endothelialization and reducing ISR.

引用

页码：87 / 96

页数：10

共 31 条

[1] Endothelial progenitor cell capture by stents coated with antibody against CD34 - The HEALING-FIM (healthy endothelial accelerated lining inhibits neointimal growth-first in man) registry [J].