H-1-NMR OF ALBUMIN IN HUMAN BLOOD-PLASMA - DRUG-BINDING AND REDOX REACTIONS AT CYS(34)

被引：84
作者：
CHRISTODOULOU, J [1 ]
SADLER, PJ [1 ]
TUCKER, A [1 ]
机构：
[1] UNIV LONDON,BIRKBECK COLL,DEPT CHEM,LONDON WC1H 0PP,ENGLAND
来源：
FEBS LETTERS | 1995年 / 376卷 / 1-2期
基金：
英国生物技术与生命科学研究理事会;
关键词：
BLOOD PLASMA; ALBUMIN; H-1; NMR; GOLD DRUG; DISULFIDE; THIOL;
D O I：
10.1016/0014-5793(95)01231-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
H-1 NMR methods are described which allow direct studies of the Cys(34) binding site of albumin in intact human blood plasma in vitro, Antiarthritic gold drugs and the alcohol-aversive drug disulfiram induce a structural transition detectable via H epsilon 1 and H delta 2 resonances of His(3) of albumin, and reactions of cystine, glutathione and captopril in plasma have also been investigated, Contrary to most assumptions, little of the albumin in normal plasma appears to be blocked at Cys(34) as a cystine disulfide.
引用
收藏
页码：1 / 5
页数：5
相关论文
共 33 条
[21]  
PEDERSEN AO, 1980, EUR J BIOCHEM, V106, P291
[22]   SERUM-ALBUMIN [J].
PETERS, T .
ADVANCES IN PROTEIN CHEMISTRY, 1985, 37 :161-245
[23]  
PETERS T, 1975, PLASMA PROTEINS, P133
[24]   GRADIENT-TAILORED EXCITATION FOR SINGLE-QUANTUM NMR-SPECTROSCOPY OF AQUEOUS-SOLUTIONS [J].
PIOTTO, M ;
SAUDEK, V ;
SKLENAR, V .
JOURNAL OF BIOMOLECULAR NMR, 1992, 2 (06) :661-665
[25]   PROTON NMR-SPECTROSCOPY - OF HUMAN-BLOOD PLASMA AND RED BLOOD-CELLS [J].
RABENSTEIN, DL ;
MILLIS, KK ;
STRAUSS, EJ .
ANALYTICAL CHEMISTRY, 1988, 60 (24) :A1380-A1391
[26]  
ROBERTS JR, 1994, 3RD INT C GOLD SILV
[27]   INVOLVEMENT OF A LYSINE RESIDUE IN THE N-TERMINAL NI2+ AND CU2+ BINDING-SITE OF SERUM ALBUMINS COMPARISON WITH CO2+, CD2+ AND AL3+ [J].
SADLER, PJ ;
TUCKER, A ;
VILES, JH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 220 (01) :193-200
[28]   PH-INDUCED STRUCTURAL TRANSITIONS OF BOVINE SERUM-ALBUMIN - HISTIDINE PKA VALUES AND UNFOLDING OF THE N-TERMINUS DURING THE N TO F TRANSITION [J].
SADLER, PJ ;
TUCKER, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (03) :811-817
[29]   PROTON NMR-STUDIES OF BOVINE SERUM-ALBUMIN - ASSIGNMENT OF SPIN SYSTEMS [J].
SADLER, PJ ;
TUCKER, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (02) :631-643
[30]  
Shaw C. F, 1989, COMMENTS INORG CHEM, V8, P233, DOI DOI 10.1080/02603598908035798
1234