RESTRICTED MOBILITY OF THE SOLE TRYPTOPHAN IN MEMBRANE-HOUND MELITTIN

被引：48
作者：
CHATTOPADHYAY, A
RUKMINI, R
机构：
[1] Centre for Cellular and Molecular Biology, Hyderabad, 500 007, Uppal Road
来源：
FEBS LETTERS | 1993年 / 335卷 / 03期
关键词：
RESTRICTED TRYPTOPHAN ENVIRONMENT; MEMBRANE-BOUND MELITTIN; RED EDGE EXCITATION SHIFT; FLUORESCENCE POLARIZATION;
D O I：
10.1016/0014-5793(93)80415-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In spite of numerous studies, there appears to be no consensus regarding the orientation and aggregation slate of membrane-bound melittin. We report here the restricted environment of the sole tryptophan residue in membrane-bound melittin using environment-induced effects on the rates of solvent relaxation. When incorporated into unilamellar vesicles of dioleoyl-sn-glycero-3-phosphocholine (DPC), melittin exhibits a red edge excitation shift (REES) of 5 nm. In addition, fluorescence polarization of melittin in the membrane shows both excitation and emission wavelength dependence. Taken together, these observations indicate that the tryptophan residue of melittin is located in a motionally restricted region in the membrane.
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页码：341 / 344
页数：4
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