DEOXYINOSINE-CONTAINING PRIMERS FOR SOLID-PHASE, AUTOMATED DNA-SEQUENCING

We developed an original strategy to perform solid-phase sequencing of an amplified bacterial gene, Design of sequencing primers was based on the consensus sequences of related genes in different bacterial species, Ambiguities were resolved using inosine-containing primers instead of degenerate primers as previously used. The data reported herein show that such deoxyinosine-containing primers can be used for direct, solid-phase automated sequencing, for up to 3 deoxyinosine residues for a 20-mer primer. (C) 1995 Wiley-Liss, Inc.