CHARACTERIZATION OF A MULTICOPY GENE FOR A MAJOR STAGE-SPECIFIC CYSTEINE PROTEINASE OF LEISHMANIA-MEXICANA

被引：103

作者：

SOUZA, AE

WAUGH, S

COOMBS, GH

MOTTRAM, JC

机构：

[1] UNIV GLASGOW,INST INFECT DIS,WELLCOME UNIT MOLEC PARASITOL,CHURCH ST,GLASGOW G11 5JS,SCOTLAND

[2] UNIV GLASGOW,DEPT ZOOL,BIOCHEM PARASITOL LAB,GLASGOW G12 8QQ,SCOTLAND

来源：

FEBS LETTERS | 1992年 / 311卷 / 02期

基金：

英国惠康基金;

关键词：

LEISHMANIA-MEXICANA; CYSTEINE PROTEINASE; AMASTIGOTE-SPECIFIC; DEVELOPMENTAL REGULATION;

D O I：

10.1016/0014-5793(92)81382-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

lmcpb, a gene from Leishmania mexicana that encodes a major cysteine proteinase in the parasite, has been cloned and sequenced. LmCPb is related more to cysteine proteinases from Trypanosoma brucei and Trypanosoma cruzi than to a previously characterized cysteine proteinase, LmCPa, of L. mexicana. It contains a long C-terminal extension characteristic of similar enzymes of T. brucei and T. cruzi. The gene is multi-copy and tandemly arranged. lmcpb RNA levels are developmentally regulated with steady state levels being high in amastigotes, low in metacyclic promastigotes and undetectable in multiplicative promastigotes. This variation correlates with and may account for the stage-specific expression of LmCPb enzyme activity.

引用

页码：124 / 127

页数：4

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